酿酒酵母中核糖体DNA的修复：RNA聚合酶I转录基因的不依赖RAD4的链特异性核苷酸切除修复
Repair of rDNA in Saccharomyces cerevisiae: RAD4-independent strand-specific nucleotide excision repair of RNA polymerase I transcribed genes.
作者信息
Verhage R A, Van de Putte P, Brouwer J
机构信息
Laboratory of Molecular Genetics, Leiden Institute of Chemistry, Leiden University, The Netherlands.
出版信息
Nucleic Acids Res. 1996 Mar 15;24(6):1020-5. doi: 10.1093/nar/24.6.1020.
Abstract
Removal of UV-induced pyrimidine dimers from the individual strands of the rDNA locus in Saccharomyces cerevisiae was studied. Yeast rDNA, that is transcribed by RNA polymerase I(RNA pol I), is repaired efficiently, slightly strand-specific and independently of RAD26, which has been implicated in transcription-coupled repair of the RNA pol II transcribed RPB2 gene. No repair of rDNA is observed in rad1,2,3 and 14 mutants, demonstrating that dimer removal from this highly repetitive DNA is accomplished by nucleotide excision repair (NER). In rad7 and rad16 mutants, which are specifically deficient in repair of non-transcribed DNA, there is a clear preferential repair of the transcribed strand of rDNA, indicating that strand-specific and therefore probably transcription-coupled repair of RNA pol I transcribed genes does exist in yeast. Unexpectedly, the transcribed but not the non-transcribed strand of rDNA can be repaired in rad4 mutants, which seem otherwise completely NER-deficient.
摘要
研究了酿酒酵母rDNA基因座单链上紫外线诱导的嘧啶二聚体的去除情况。酵母rDNA由RNA聚合酶I（RNA pol I）转录，其修复效率高，具有轻微的链特异性，且独立于RAD26，而RAD26参与RNA聚合酶II转录的RPB2基因的转录偶联修复。在rad1、2、3和14突变体中未观察到rDNA的修复，这表明从这种高度重复的DNA中去除二聚体是通过核苷酸切除修复（NER）完成的。在rad7和rad16突变体中，它们在非转录DNA修复方面存在特异性缺陷，rDNA的转录链有明显的优先修复，这表明酵母中确实存在RNA聚合酶I转录基因的链特异性以及可能的转录偶联修复。出乎意料的是，在rad4突变体中，rDNA的转录链而非非转录链可以被修复，而rad4突变体在其他方面似乎完全缺乏核苷酸切除修复能力。
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本文引用的文献
1
Stranded in an active gene.被困在一个活跃基因中。
Curr Biol. 1993 Jan;3(1):67-9. doi: 10.1016/0960-9822(93)90156-i.
2
Double mutants of Saccharomyces cerevisiae with alterations in global genome and transcription-coupled repair.酿酒酵母在全基因组和转录偶联修复方面发生改变的双突变体。
Mol Cell Biol. 1996 Feb;16(2):496-502. doi: 10.1128/MCB.16.2.496.
3
Chromatin structures and transcription of rDNA in yeast Saccharomyces cerevisiae.酿酒酵母中rDNA的染色质结构与转录
Nucleic Acids Res. 1993 May 25;21(10):2331-8. doi: 10.1093/nar/21.10.2331.
4
Molecular mechanism of transcription-repair coupling.转录修复偶联的分子机制。
Science. 1993 Apr 2;260(5104):53-8. doi: 10.1126/science.8465200.
5
Lack of transcription-coupled repair in mammalian ribosomal RNA genes.哺乳动物核糖体RNA基因中缺乏转录偶联修复。
Biochemistry. 1993 Oct 5;32(39):10512-8. doi: 10.1021/bi00090a030.
6
Deficient repair of the transcribed strand of active genes in Cockayne's syndrome cells.科凯恩综合征细胞中活性基因转录链修复缺陷。
Nucleic Acids Res. 1993 Dec 25;21(25):5890-5. doi: 10.1093/nar/21.25.5890.
7
Yeast nucleotide excision repair proteins Rad2 and Rad4 interact with RNA polymerase II basal transcription factor b (TFIIH).酵母核苷酸切除修复蛋白Rad2和Rad4与RNA聚合酶II基础转录因子b（TFIIH）相互作用。
Mol Cell Biol. 1994 Jun;14(6):3569-76. doi: 10.1128/mcb.14.6.3569-3576.1994.
8
Purification and cloning of a nucleotide excision repair complex involving the xeroderma pigmentosum group C protein and a human homologue of yeast RAD23.一种涉及着色性干皮病C组蛋白和酵母RAD23人类同源物的核苷酸切除修复复合物的纯化与克隆
EMBO J. 1994 Apr 15;13(8):1831-43. doi: 10.1002/j.1460-2075.1994.tb06452.x.
9
DNA repair genes and proteins of Saccharomyces cerevisiae.酿酒酵母的DNA修复基因与蛋白质
Annu Rev Genet. 1993;27:33-70. doi: 10.1146/annurev.ge.27.120193.000341.
10
DNA repair defect in xeroderma pigmentosum group C and complementing factor from HeLa cells.着色性干皮病C组中的DNA修复缺陷及来自HeLa细胞的互补因子。
J Biol Chem. 1994 Sep 9;269(36):22749-57.
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