阿尔茨海默病中低分子量酸性磷酸酶蛋白水平的改变。

Alterations of low molecular weight acid phosphatase protein level in Alzheimer's disease.

作者信息

Shimohama S, Fujimoto S, Chachin M, Taniguchi T, Perry G, Whitehouse P J, Kimura J

机构信息

Department of Neurology, Faculty of Medicine, Kyoto University, Japan.

出版信息

Brain Res. 1995 Nov 13;699(1):125-9. doi: 10.1016/0006-8993(95)00995-3.

DOI:10.1016/0006-8993(95)00995-3

PMID:8616601

Abstract

We have previously reported that the activity of low molecular weight (LMW) acid phosphatase, which can remove tyrosine-linked phosphates of epidermal growth factor receptor, was significantly decreased in Alzheimer brains. In the present study, a specific antibody was prepared to analyze the protein level of this enzyme. Western blot analysis indicated that the level of LMW acid phosphatase protein was significantly reduced, whereas the activity of LMW acid phosphatase per enzyme molecule was not changed in Alzheimer brains. These results suggest that the reduction of LMW acid phosphatase activity in Alzheimer brains is due to its decreased protein level in Alzheimer's disease.

摘要

我们之前曾报道，能够去除表皮生长因子受体酪氨酸连接磷酸基团的低分子量（LMW）酸性磷酸酶的活性，在阿尔茨海默病大脑中显著降低。在本研究中，制备了一种特异性抗体来分析这种酶的蛋白质水平。蛋白质印迹分析表明，LMW酸性磷酸酶蛋白水平显著降低，而在阿尔茨海默病大脑中每个酶分子的LMW酸性磷酸酶活性并未改变。这些结果表明，阿尔茨海默病大脑中LMW酸性磷酸酶活性的降低是由于其在阿尔茨海默病中的蛋白质水平下降所致。