Oh C K, Metcalfe D D
Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
Biochem Biophys Res Commun. 1996 Apr 25;221(3):510-4. doi: 10.1006/bbrc.1996.0627.
Aggregates of mast cells and lymphocytes have been found in inflamed tissues suggesting that lymphocytes may have the ability to activate mast cells through cell-to-cell contact. To examine this hypothesis, murine mast cells were transfected with a T cell activation gene-3 (TCA3)-chloramphenicol acetyl transferase (CAT) construct, and these cells co-cultured with murine EL-4 (T), CH12.LX (B), WEHI-3 (myelomonocytic) or 3T3(fibroblast) cell lines. Co-culture of activated EL-4 or CH12.LX cells, but not WEHI-3 or 3T3 cells, with transfected mast cells induced a 5 to 7 fold increase in CAT expression which was dependent on the lymphocyte to mast cell ratio. Supernatants from activated EL-4 or CH12.LX cells did not induce CAT expression in transfected mast cells. These data demonstrate that activated lymphocytes have the ability to induce the promoter of the TCA3 gene in mast cells through a mechanism requiring cell-to-cell contact, and suggest the possibility that activated lymphocytes may effect other biologic processes in mast cells as well through such heterotypic activation.
在炎症组织中发现了肥大细胞和淋巴细胞的聚集物，这表明淋巴细胞可能具有通过细胞间接触激活肥大细胞的能力。为了验证这一假设，用T细胞激活基因-3（TCA3）-氯霉素乙酰转移酶（CAT）构建体转染小鼠肥大细胞，并将这些细胞与小鼠EL-4（T）、CH12.LX（B）、WEHI-3（骨髓单核细胞）或3T3（成纤维细胞）细胞系共培养。活化的EL-4或CH12.LX细胞（而非WEHI-3或3T3细胞）与转染的肥大细胞共培养，可使CAT表达增加5至7倍，这取决于淋巴细胞与肥大细胞的比例。活化的EL-4或CH12.LX细胞的上清液不会诱导转染的肥大细胞中CAT表达。这些数据表明，活化的淋巴细胞能够通过一种需要细胞间接触的机制诱导肥大细胞中TCA3基因的启动子，这也提示活化的淋巴细胞可能通过这种异型激活影响肥大细胞中的其他生物学过程。
