通过毛细管等速电泳预浓缩的核糖核苷酸的在线拉曼光谱分析。

On-line Raman spectroscopy of ribonucleotides preconcentrated by capillary isotachophoresis.

作者信息

Walker P A, Kowalchyk W K, Morris M D

机构信息

Department of Chemistry, University of Michigan, Ann Arbor 48109-1055, USA.

出版信息

Anal Chem. 1995 Dec 1;67(23):4255-60. doi: 10.1021/ac00119a009.

DOI:10.1021/ac00119a009

PMID:8633770

Abstract

Normal Raman spectroscopy is used as an on-line detector for capillary isotachophoresis (ITP) of adenosine 5'-triphosphate, adenosine 5'-diphosphate, and adenosine 5'-monophosphate in phosphate buffers. Preconcentration is from a 1 x 10(-2) M phosphate buffer (pH 7.5) into a leading electrolyte of 0.1 M KCl or Na2SO4, with a terminating electrolyte of 0.1 M 4-morpholinepropane-sulfonic acid. The ribonucleotides are concentrated to above 10(-2) M at the detection window, allowing measurement of Raman spectra with 1 s integration, from starting concentrations of 5 x 10(-6) M or higher.

摘要

正常拉曼光谱用作磷酸盐缓冲液中三磷酸腺苷、二磷酸腺苷和一磷酸腺苷毛细管等速电泳（ITP）的在线检测器。预浓缩是从1×10⁻² M磷酸盐缓冲液（pH 7.5）到0.1 M KCl或Na₂SO₄的前导电解质中，终止电解质为0.1 M 4-吗啉丙烷磺酸。在检测窗口处，核糖核苷酸被浓缩至10⁻² M以上，从而能够从5×10⁻⁶ M或更高的起始浓度开始，以1秒积分时间测量拉曼光谱。