+P5（D1S3309E）是威尔姆斯肿瘤抑制基因1（WT1）的一个新型靶标结合位点，定位于人类染色体1q21→22。

+P5 (D1S3309E), a novel target binding site for the Wilms' tumour suppressor 1 (WT1) gene, maps to human chromosome 1q21-->22.

作者信息

Negus K, Holmes G H, Wicking C, Wainwright B J, Little M H

机构信息

Centre for Molecular and Cellular Biology, University of Queensland, St. Lucia, Australia.

出版信息

Cytogenet Cell Genet. 1996;72(4):306-9. doi: 10.1159/000134210.

DOI:10.1159/000134210

PMID:8641136

Abstract

The Wilms' tumor suppressor 1 gene (WT1) encodes a zinc finger transcription factor critical for normal urogenital development. We have previously isolated a DNA fragment, +P5 (D1S3309E), to which all WT1 protein isoforms bind. Using PCR of a human x rodent somatic cell hybrid mapping panel, together with two-color fluorescence in situ hybridisation of +P5-containing cosmids and previously localised human chromosome 1q cosmids, we have mapped the +P5 fragment to chromosome 1q21-->q22.

摘要

肾母细胞瘤抑制基因1（WT1）编码一种对正常泌尿生殖系统发育至关重要的锌指转录因子。我们之前分离出了一个DNA片段，即+P5（D1S3309E），所有WT1蛋白异构体均可与之结合。利用人-啮齿动物体细胞杂种定位板进行聚合酶链反应（PCR），并结合含+P5黏粒和先前定位的人类1号染色体q区黏粒的双色荧光原位杂交技术，我们已将+P5片段定位到1号染色体q21→q22区。