Hall M O, Burgess B L, Abrams T A, Martinez M O
Jules Stein Eye Institute, University of California, Los Angeles School of Medicine 90095-7008, USA.
Invest Ophthalmol Vis Sci. 1996 Jun;37(7):1473-7.
To investigate the effect of carbachol on the phagocytosis of photoreceptor outer segments (OS) in cultures of normal Long-Evans and dystrophic Royal College of Surgeons (RCS) rat retinal pigment epithelial (RPE) cells.
Retinal pigment epithelial cells from normal and RCS rats were grown in tissue culture. On reaching confluence, they were presented with OS suspended in Krebs-Henseleit buffer in the presence or absence of carbachol and LiCl. The number of bound and ingested OS was quantitated using double immunofluorescence staining.
LiCl inhibited the ingestion of OS by more than 90% but had no effect on the binding of OS by Long-Evans RPE cells. The addition of carbachol further reduced OS ingestion. Carbachol alone decreased OS ingestion by normal RPE cells by 30% but had no effect on OS binding. The effect of LiCl and carbachol on RCS RPE cells was similar to their effect on normal RPE cells.
Carbachol does not increase OS phagocytosis in normal or RCS rat RPE cells. The phagocytic defect in RCS rat RPE cannot be reversed or overcome by stimulation of the IP3 pathway by carbachol. LiCl strongly inhibits the ingestion of OS by normal and by RCS RPE cells, and this effect is enhanced by carbachol.
研究卡巴胆碱对正常长 Evans 大鼠和营养不良性皇家外科学院（RCS）大鼠视网膜色素上皮（RPE）细胞培养物中光感受器外段（OS）吞噬作用的影响。
将正常大鼠和 RCS 大鼠的视网膜色素上皮细胞进行组织培养。细胞汇合后，在存在或不存在卡巴胆碱和氯化锂的情况下，向其提供悬浮于 Krebs-Henseleit 缓冲液中的 OS。使用双重免疫荧光染色对结合和摄取的 OS 数量进行定量。
氯化锂抑制 OS 的摄取超过 90%，但对长 Evans RPE 细胞结合 OS 没有影响。添加卡巴胆碱进一步降低了 OS 的摄取。单独使用卡巴胆碱可使正常 RPE 细胞的 OS 摄取减少 30%，但对 OS 的结合没有影响。氯化锂和卡巴胆碱对 RCS RPE 细胞的作用与其对正常 RPE 细胞的作用相似。
卡巴胆碱不会增加正常或 RCS 大鼠 RPE 细胞中的 OS 吞噬作用。卡巴胆碱通过刺激 IP3 途径无法逆转或克服 RCS 大鼠 RPE 中的吞噬缺陷。氯化锂强烈抑制正常和 RCS RPE 细胞对 OS 的摄取，并且卡巴胆碱可增强这种作用。
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