肉毒杆菌神经毒素轻链可抑制钙离子诱导的以及鸟苷三磷酸类似物诱导的儿茶酚胺从透化的肾上腺嗜铬细胞中释放。

Botulinum neurotoxin light chains inhibit both Ca(2+)-induced and GTP analogue-induced catecholamine release from permeabilised adrenal chromaffin cells.

作者信息

Glenn D E, Burgoyne R D

机构信息

Physiological Laboratory, University of Liverpool, UK.

出版信息

FEBS Lett. 1996 May 20;386(2-3):137-40. doi: 10.1016/0014-5793(96)00432-2.

DOI:10.1016/0014-5793(96)00432-2

PMID:8647268

Abstract

Using digitonin-permeabilised bovine adrenal chromaffin cells, the effects of botulinum neurotoxin light chains on exocytosis triggered by Ca2+ or by GppNHp were examined. Botulinum neurotoxin D light chain, prepared as a His(6)-tagged recombinant protein, cleaved VAMP and substantially inhibited catecholamine release due to Ca2+ and GppNHp. Botulinum neurotoxin C1 and E light chains produced partial inhibition of both Ca(2+)- and GppNHp-induced catecholamine release. These results suggest that Ca(2+)-dependent exocytosis and Ca(2+)-independent exocytosis triggered by a non-hydrolysable GTP analogue occurs via a SNARE-dependent mechanism in chromaffin cells.

摘要

利用洋地黄皂苷通透处理的牛肾上腺嗜铬细胞，研究了肉毒杆菌神经毒素轻链对由Ca2+或GppNHp触发的胞吐作用的影响。作为His(6)标签重组蛋白制备的肉毒杆菌神经毒素D轻链，切割VAMP并显著抑制由Ca2+和GppNHp引起的儿茶酚胺释放。肉毒杆菌神经毒素C1和E轻链对Ca(2+)和GppNHp诱导的儿茶酚胺释放均产生部分抑制作用。这些结果表明，在嗜铬细胞中，由不可水解的GTP类似物触发的Ca(2+)依赖性胞吐作用和Ca(2+)非依赖性胞吐作用是通过SNARE依赖性机制发生的。