Kuramoto A, Lezhava A, Taguchi S, Momose H, Kinashi H
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Japan.
FEMS Microbiol Lett. 1996 May 15;139(1):37-42. doi: 10.1111/j.1574-6968.1996.tb08176.x.
The genes coding for the protease inhibitors, SSI and API-2c', have been analyzed by comparing DNA macrorestriction patterns of Streptomyces albogriseolus S-3253 and S. griseoincarnatus KTo-250 with those of inhibitor-deficient mutants. The mutants were found to suffer from chromosomal deletions rather than plasmid loss which resulted in the loss of the relevant genes. Hybridization experiments indicated that the ssi homologs in S. lividans and S. coelicolor A3(2) are located near the end of the linear chromosome.
通过比较浅灰链霉菌S-3253和肉色链霉菌KTo-250与抑制剂缺陷型突变体的DNA宏观限制性图谱，对编码蛋白酶抑制剂SSI和API-2c'的基因进行了分析。发现这些突变体存在染色体缺失而非质粒丢失，这导致了相关基因的丧失。杂交实验表明，天蓝色链霉菌和变铅青链霉菌A3(2)中的ssi同源物位于线性染色体的末端附近。
