Motazedian H, Noyes H, Maingon R
Liverpool School of Tropical Medicine, United Kingdom.
Exp Parasitol. 1996 Jun;83(1):150-4. doi: 10.1006/expr.1996.0059.
We used the RAPD-PCR method for distinguishing the main Old World Leishmania parasites Leishmania tropica, L. major, and L. infantum and applied it to Sauroleishmania species from Iran. Twelve out of 21 tested primers were suitable for identification of these parasites. The Jaccard similarity index was 0.30 for L. tropica and L. infantum as well as for L. major and L. infantum. The similarity coefficient for L. major and L. infantum was 0.22 and for L. tropica and L. major it was 0.26. These data agree well with established phylogenetic/ taxonomic classification. The index between different isolates derived from various hosts and sandfly vectors for the same Leishmania species was 1, indicating that this method is suitable for epidemiological analysis.
我们使用随机扩增多态性DNA聚合酶链反应（RAPD-PCR）方法来区分主要的旧大陆利什曼原虫寄生虫——热带利什曼原虫、硕大利什曼原虫和婴儿利什曼原虫，并将其应用于来自伊朗的蜥蜴利什曼原虫物种。21种测试引物中有12种适用于这些寄生虫的鉴定。热带利什曼原虫和婴儿利什曼原虫以及硕大利什曼原虫和婴儿利什曼原虫的杰卡德相似性指数为0.30。硕大利什曼原虫和婴儿利什曼原虫的相似系数为0.22，热带利什曼原虫和硕大利什曼原虫的相似系数为0.26。这些数据与既定的系统发育/分类学分类非常吻合。来自同一利什曼原虫物种的不同宿主和白蛉载体的不同分离株之间的指数为1，表明该方法适用于流行病学分析。
