HIV-1的巨噬细胞嗜性和非巨噬细胞嗜性分离株与胸腺和扁桃体树突状细胞在体外的相互作用。
The interaction of macrophage and non-macrophage tropic isolates of HIV-1 with thymic and tonsillar dendritic cells in vitro.
作者信息
Cameron P U, Lowe M G, Sotzik F, Coughlan A F, Crowe S M, Shortman K
机构信息
AIDS Pathogenesis Unit, Macfarlane Burnet Centre for Medical Research, Fairfield Hospital, Victoria, Australia.
出版信息
J Exp Med. 1996 Apr 1;183(4):1851-6. doi: 10.1084/jem.183.4.1851.
Abstract
Dendritic cells isolated from thymus and tonsil were tested for susceptibility to HIV-1 strains that are tropic for macrophages or for T cell lines. DCs were purified by cell sorting and before infection expressed high levels of CD4 and HLA-DR and lacked markers for T, B, NK cells, or macrophages. Viral entry and reverse transcription was found after pulsing with strains of HIV-1 that could infect macrophages. During the first 36 h the PCR signals for gag sequences increased in DCs and macrophages. In contrast little if any viral DNA was found after pulsing macrophages or DCs with HIV-1 that was able to infect T cell lines. DCs pulsed with HIV-1 were able to transmit infection to responding T cells during an allogeneic or superantigen response. Selection for virus able to infect lymphoid DCs and other DCs expressing CD4 and its transfer to T cells during subsequent immune responses may provide a mechanism for the observed predominance of macrophage-tropic HIV-1 after in vivo transmission.
摘要
对从胸腺和扁桃体分离出的树突状细胞进行检测，以确定其对嗜巨噬细胞或嗜T细胞系的HIV-1毒株的易感性。通过细胞分选纯化树突状细胞，在感染前，其表达高水平的CD4和HLA-DR，且缺乏T细胞、B细胞、NK细胞或巨噬细胞的标志物。在用可感染巨噬细胞的HIV-1毒株脉冲处理后，发现了病毒进入和逆转录现象。在最初的36小时内，树突状细胞和巨噬细胞中gag序列的PCR信号增加。相比之下，在用能够感染T细胞系的HIV-1脉冲处理巨噬细胞或树突状细胞后，几乎未发现病毒DNA。用HIV-1脉冲处理的树突状细胞能够在同种异体或超抗原反应期间将感染传递给反应性T细胞。选择能够感染淋巴树突状细胞和其他表达CD4的树突状细胞的病毒，并在随后的免疫反应中将其传递给T细胞，这可能为体内传播后观察到的嗜巨噬细胞HIV-1占优势提供一种机制。
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本文引用的文献
1
Thymic dendritic cells and T cells develop simultaneously in the thymus from a common precursor population.胸腺树突状细胞和T细胞在胸腺中由共同的前体细胞群同时发育而来。
Nature. 1993 Apr 22;362(6422):761-3. doi: 10.1038/362761a0.
2
B7/BB-1 is a leucocyte differentiation antigen on human dendritic cells induced by activation.B7/BB-1是人类树突状细胞经激活后诱导产生的一种白细胞分化抗原。
Immunology. 1993 Aug;79(4):616-20.
3
Human immunodeficiency virus infection of the human thymus and disruption of the thymic microenvironment in the SCID-hu mouse.人类胸腺的人类免疫缺陷病毒感染及SCID-hu小鼠胸腺微环境的破坏。
J Exp Med. 1993 Oct 1;178(4):1151-63. doi: 10.1084/jem.178.4.1151.
4
Genotypic and phenotypic characterization of HIV-1 patients with primary infection.初次感染HIV-1患者的基因和表型特征
Science. 1993 Aug 27;261(5125):1179-81. doi: 10.1126/science.8356453.
5
CD4-independent infection of human peripheral blood dendritic cells with isolates of human immunodeficiency virus type 1.1型人类免疫缺陷病毒分离株对人外周血树突状细胞的不依赖CD4感染。
J Gen Virol. 1993 Jul;74 ( Pt 7):1277-85. doi: 10.1099/0022-1317-74-7-1277.
6
Dendritic cells freshly isolated from human blood express CD4 and mature into typical immunostimulatory dendritic cells after culture in monocyte-conditioned medium.从人血液中新鲜分离的树突状细胞表达CD4，并在单核细胞条件培养基中培养后成熟为典型的免疫刺激性树突状细胞。
J Exp Med. 1993 Sep 1;178(3):1067-76. doi: 10.1084/jem.178.3.1067.
7
CD4 and CD8 expression by human and mouse thymic dendritic cells.
Immunol Lett. 1994 May;40(2):93-9. doi: 10.1016/0165-2478(94)90178-3.
8
Susceptibility of dendritic cells to HIV-1 infection in vitro.
J Leukoc Biol. 1994 Sep;56(3):257-65. doi: 10.1002/jlb.56.3.257.
9
Infection and apoptotic cell death of CD4+ T cells during an immune response to HIV-1-pulsed dendritic cells.
AIDS Res Hum Retroviruses. 1994 Jan;10(1):61-71. doi: 10.1089/aid.1994.10.61.
10
Three populations of cells with dendritic morphology exist in peripheral blood, only one of which is infectable with human immunodeficiency virus type 1.外周血中存在三种具有树突状形态的细胞群，其中只有一种可被1型人类免疫缺陷病毒感染。
Proc Natl Acad Sci U S A. 1995 Jan 31;92(3):826-30. doi: 10.1073/pnas.92.3.826.
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