Cameron P U, Lowe M G, Sotzik F, Coughlan A F, Crowe S M, Shortman K
AIDS Pathogenesis Unit, Macfarlane Burnet Centre for Medical Research, Fairfield Hospital, Victoria, Australia.
J Exp Med. 1996 Apr 1;183(4):1851-6. doi: 10.1084/jem.183.4.1851.
Dendritic cells isolated from thymus and tonsil were tested for susceptibility to HIV-1 strains that are tropic for macrophages or for T cell lines. DCs were purified by cell sorting and before infection expressed high levels of CD4 and HLA-DR and lacked markers for T, B, NK cells, or macrophages. Viral entry and reverse transcription was found after pulsing with strains of HIV-1 that could infect macrophages. During the first 36 h the PCR signals for gag sequences increased in DCs and macrophages. In contrast little if any viral DNA was found after pulsing macrophages or DCs with HIV-1 that was able to infect T cell lines. DCs pulsed with HIV-1 were able to transmit infection to responding T cells during an allogeneic or superantigen response. Selection for virus able to infect lymphoid DCs and other DCs expressing CD4 and its transfer to T cells during subsequent immune responses may provide a mechanism for the observed predominance of macrophage-tropic HIV-1 after in vivo transmission.
对从胸腺和扁桃体分离出的树突状细胞进行检测,以确定其对嗜巨噬细胞或嗜T细胞系的HIV-1毒株的易感性。通过细胞分选纯化树突状细胞,在感染前,其表达高水平的CD4和HLA-DR,且缺乏T细胞、B细胞、NK细胞或巨噬细胞的标志物。在用可感染巨噬细胞的HIV-1毒株脉冲处理后,发现了病毒进入和逆转录现象。在最初的36小时内,树突状细胞和巨噬细胞中gag序列的PCR信号增加。相比之下,在用能够感染T细胞系的HIV-1脉冲处理巨噬细胞或树突状细胞后,几乎未发现病毒DNA。用HIV-1脉冲处理的树突状细胞能够在同种异体或超抗原反应期间将感染传递给反应性T细胞。选择能够感染淋巴树突状细胞和其他表达CD4的树突状细胞的病毒,并在随后的免疫反应中将其传递给T细胞,这可能为体内传播后观察到的嗜巨噬细胞HIV-1占优势提供一种机制。