Cunliffe N A, Fergusson S, Davidson F, Lyon A, Ross P W
Department of Medical Microbiology, University of Edinburgh, Medical School, UK.
J Med Microbiol. 1996 Jul;45(1):27-30. doi: 10.1099/00222615-45-1-27.
Polymerase chain reaction (PCR) amplification of the urease genes of Ureaplasma urealyticum was compared with culture for detection of the organism in 100 endotracheal aspirates from 54 ventilated preterm infants. Ninety specimens gave negative results by both culture and PCR and three specimens gave positive results by both culture and PCR. Six specimens were negative by culture but positive by PCR. The one specimen positive by culture and negative by PCR was interpreted as a false-positive culture result. Overall agreement between results obtained by culture and PCR was 93%. PCR is a sensitive and reliable method for the detection of U. urealyticum in neonatal endotracheal secretions. Detection by PCR (1-2 days) is more rapid than culture (2-5 days) and this will be important if early therapeutic intervention is shown to be effective.
对54例机械通气的早产儿的100份气管内吸出物进行解脲脲原体脲酶基因的聚合酶链反应（PCR）扩增，并与培养法检测该病原体的结果进行比较。90份标本培养和PCR检测均为阴性，3份标本培养和PCR检测均为阳性。6份标本培养阴性但PCR检测阳性。1份标本培养阳性但PCR检测阴性，被判定为培养假阳性结果。培养和PCR结果的总体一致性为93%。PCR是检测新生儿气管内分泌物中解脲脲原体的一种灵敏且可靠的方法。PCR检测（1 - 2天）比培养法（2 - 5天）更快，如果早期治疗干预被证明有效，这一点将很重要。
