黑曲霉无纤维素酶木聚糖酶的生产、特性及应用

Production, characterization and application of the cellulase-free xylanase from Aspergillus niger.

作者信息

Qy Y, Gao P, Wang D, Zhao X, Zhang X

机构信息

Institute of Microbiology, Shandong University, Jinan, P.R. China.

出版信息

Appl Biochem Biotechnol. 1996 Spring;57-58:375-81. doi: 10.1007/BF02941716.

DOI:10.1007/BF02941716

PMID:8669904

Abstract

The effects of carbon source on xylanase and cellulase production were studied. The extract of steam-exploded corn stover was found to be the best raw material for producing cellulase-free xylanase. The xylanolytic enzymes of Aspergillus niger An-76 were purified by chromatography, and the properties of the four purified components were analyzed. When the enzyme was used to treat the birch Kraft pulp, and followed by a subsequent CEH bleaching, a brightness of 6.8% SBD more than that of the untreated one using the same chlorine dosage, or a saving of nearly 50% of chlorine consumption with the same brightness, was realized.

摘要

研究了碳源对木聚糖酶和纤维素酶产生的影响。发现蒸汽爆破玉米秸秆提取物是生产无纤维素酶木聚糖酶的最佳原料。通过色谱法对黑曲霉An-76的木聚糖分解酶进行了纯化，并分析了四种纯化组分的性质。当该酶用于处理桦木硫酸盐浆，随后进行CEH漂白时，在相同氯用量下，亮度比未处理的纸浆提高了6.8% SBD，或者在相同亮度下，氯消耗量节省了近50%。