大鼠嗅觉上皮的45 kDa GTP结合蛋白：纯化、特性鉴定及定位

45-kDa GTP-binding protein from rat olfactory epithelium: purification, characterization and localization.

作者信息

Novoselov V I, Peshenko I V, Evdokimov V A, Nikolaev J V, Matveeva E A, Fesenko E E

机构信息

Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.

出版信息

Chem Senses. 1996 Apr;21(2):181-8. doi: 10.1093/chemse/21.2.181.

DOI:10.1093/chemse/21.2.181

PMID:8670696

Abstract

The rat olfactory epithelium contains a specific water-soluble 45-kDa protein. This protein is recognized by anti-peptide antibodies which react with alpha-subunits of the known G-proteins. The 45-kDa protein has been isolated using DEAE-chromatography and gel-exclusion chromatography. The content of 45-kDa protein is about 2% of the total soluble proteins of the olfactory mucosa and it is located at the mucociliary surface. According to photo-affinity labeling, the 45-kDa protein possesses a high affinity to GTP and exhibits low GTP hydrolytic activity. The functions of the 45-kDa protein are discussed.

摘要

大鼠嗅觉上皮含有一种特定的水溶性45 kDa蛋白质。这种蛋白质可被与已知G蛋白的α亚基发生反应的抗肽抗体识别。已使用DEAE柱层析和凝胶排阻层析法分离出45 kDa蛋白质。45 kDa蛋白质的含量约占嗅觉黏膜总可溶性蛋白质的2%，且位于黏液纤毛表面。根据光亲和标记法，45 kDa蛋白质对GTP具有高亲和力，并表现出低GTP水解活性。文中讨论了45 kDa蛋白质的功能。