Migliore L, Cocchi L, Scarpato R
Dipartimento di Scienze dell'Ambiente e del Territorio, Università di Pisa, Via S Giuseppe 22, 56100 Pisa, Italy.
Mutagenesis. 1996 May;11(3):285-90. doi: 10.1093/mutage/11.3.285.
The human lymphocyte micronucleus (MN) test combined with fluorescence in situ hybridization (FISH) of a centromeric probe is considered a useful screening assay to distinguish between clastogenic and aneugenic agents. Four suspected aneuploidy-inducing chemicals, acetaldehyde (AA), diethylstilbestrol (DES), diethylstilbestrol dipropionate (DESdp) and griseofulvin (GF), have been evaluated with the assay. All compounds induced a significant increase of MN at all doses tested. After the application of the FISH technique with a pancentromeric DNA sequence, DES, DESdp and GF showed a statistically significant increase in the percentage of positive signals compared with the control culture. GF induced the highest percentage of centromere-positive MN observed to date (>90% on average). AA did not show a significant difference in the percentage of centromere-positive MN. The results indicate that in human lymphocytes DES, DESdp and GF act primarily as aneugens, while AA seems capable of causing both chromosome breakage and aneuploidy.
人类淋巴细胞微核（MN）试验结合着丝粒探针的荧光原位杂交（FISH）被认为是一种区分致断裂剂和非整倍体诱导剂的有用筛选试验。已用该试验评估了四种疑似非整倍体诱导化学物质，即乙醛（AA）、己烯雌酚（DES）、己烯雌酚二丙酸酯（DESdp）和灰黄霉素（GF）。所有化合物在所有测试剂量下均诱导MN显著增加。应用全着丝粒DNA序列的FISH技术后，与对照培养相比，DES、DESdp和GF的阳性信号百分比有统计学显著增加。GF诱导的着丝粒阳性MN百分比是迄今为止观察到的最高值（平均>90%）。AA在着丝粒阳性MN百分比方面未显示出显著差异。结果表明，在人类淋巴细胞中，DES、DESdp和GF主要作为非整倍体诱导剂起作用，而AA似乎既能导致染色体断裂，又能导致非整倍体。
