一种使用放射性标记的合成羟基磷灰石评估口腔细菌致龋性的方法。
A method for evaluating the cariogenicity of oral bacteria using radio-labelled synthetic hydroxyapatite.
作者信息
Yamaguchi R, Sato M, Tsuchiya H, Yamamoto K, Doi Y, Iwaku F
机构信息
Research Institute of Radioisotope, Asahi University School of Dentistry, Gifu, Japan.
出版信息
Lett Appl Microbiol. 1996 May;22(5):375-7. doi: 10.1111/j.1472-765x.1996.tb01182.x.
Radioactive hydroxyapatite was synthesized using 45Ca to evaluate the cariogenicity of oral streptococci. Discs prepared from it were suspended in media containing sucrose, then inoculated with Streptococcus mutans or Streptococcus sanguis. The radioactivity in the supernatant fluid was measured at specified time intervals. Released 45Ca in the supernatant fluids markedly increased in both species during the experimental period, while Strep. mutans showed much higher decalcification than Strep. sanguis. The present method would be useful for semi-quantitative evaluation of bacterial decalcification ability.
使用45Ca合成放射性羟基磷灰石，以评估口腔链球菌的致龋性。由其制备的圆盘悬浮于含蔗糖的培养基中，然后接种变形链球菌或血链球菌。在特定时间间隔测量上清液中的放射性。在实验期间，两种菌的上清液中释放的45Ca均显著增加，而变形链球菌的脱钙程度远高于血链球菌。本方法可用于细菌脱钙能力的半定量评估。
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