氧化剂介导的肺平滑肌微粒体中Ca(+) -ATP酶的蛋白水解激活作用
Oxidant-mediated proteolytic activation of Ca(+)-ATPase in microsomes of pulmonary smooth muscle.
作者信息
Ghosh S K, Chakraborti T, Michael J R, Chakraborti S
机构信息
Department of Biochemistry and Biophysics, University of Kalyani, India.
出版信息
FEBS Lett. 1996 Jun 3;387(2-3):171-4. doi: 10.1016/0014-5793(96)00471-1.
Treatment of bovine pulmonary artery smooth muscle tissue microsomes with H2O2 (1 mM) markedly stimulated protease activity tested with a synthetic substrate N-benzoyl-DL-arginine p-nitroanilide (BAPNA), and also enhanced Ca(2+)-ATPase activity. ATP-dependent Ca(2+) uptake was found to be stimulated upon treatment of the microsomes with H2O2. Pretreatment of the microsomes with vitamin E and aprotinin prevented the H2O2-induced stimulation of Ca(2+)-ATPase activity and also ATP-dependent Ca(2+) uptake. In contrast, H2O2-induced inhibition of Na(+)-dependent Ca(2+) uptake was reversed by vitamin E and aprotinin.
用H2O2（1 mM）处理牛肺动脉平滑肌组织微粒体，可显著刺激用合成底物N-苯甲酰-DL-精氨酸对硝基苯胺（BAPNA）检测的蛋白酶活性，同时也增强Ca(2+)-ATP酶活性。发现用H2O2处理微粒体后，ATP依赖的Ca(2+)摄取受到刺激。用维生素E和抑肽酶预处理微粒体可防止H2O2诱导的Ca(2+)-ATP酶活性刺激以及ATP依赖的Ca(2+)摄取。相反，维生素E和抑肽酶可逆转H2O2诱导的Na(+)-依赖的Ca(2+)摄取抑制。
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