Sabra M C, Jørgensen K, Mouritsen O G
Department of Physical Chemistry, Technical University of Denmark, Lyngby, Denmark.
Biochim Biophys Acta. 1996 Jun 13;1282(1):85-92. doi: 10.1016/0005-2736(96)00042-9.
The effects of a small molecule, the insecticide lindane, on unilamellar DMPC bilayers in the phase transition region, have been studied by means of differential scanning calorimetry and fluorescence spectroscopy. The calorimetric data show that increasing concentrations of lindane broaden the transition and lower the transition temperature, without changing the transition enthalpy significantly. Lindane therefore enhances the thermal fluctuations of the bilayer. The calorimetric data furthermore suggest that the bilayer structure is intact and not disrupted by even high concentrations (32 mol%) of lindane. Fluorescence spectroscopy was used to measure the passive permeability of unilamellar DMPC bilayers to Co2+ ions. The data show that lindane seals the bilayer for Co2+ penetration and that this effect increases with increasing lindane concentration. The results are discussed in relation to the effects on the permeability of other small molecules, e.g., anesthetics.
通过差示扫描量热法和荧光光谱法研究了小分子杀虫剂林丹对处于相变区域的单层二肉豆蔻酰磷脂酰胆碱（DMPC）双层膜的影响。量热数据表明，林丹浓度增加会使转变变宽并降低转变温度，但转变焓没有显著变化。因此，林丹增强了双层膜的热涨落。量热数据还表明，即使在高浓度（32摩尔%）的林丹作用下，双层膜结构依然完整，未被破坏。利用荧光光谱法测量了单层DMPC双层膜对Co2+离子的被动通透性。数据表明，林丹可封闭双层膜以阻止Co2+渗透，且这种效应随林丹浓度的增加而增强。结合林丹对其他小分子（如麻醉剂）通透性的影响对结果进行了讨论。
