Göhring U J, Scharl A, Ahr A
Klinik und Poliklinik für Frauenheilkunde und Geburtshilfe, Universitt Köln.
Geburtshilfe Frauenheilkd. 1996 Apr;56(4):177-83. doi: 10.1055/s-2007-1022256.
We tested whether immunohistochemical detection of oestrogen and progesterone receptor (ER, PR), the oestrogen-dependent protein pS2, the growth hormone receptors p 185neu and EGF-R, the tumour suppressor protein p53, the tissue proteases Cathepsin D and Urokinase, and the proliferation marker PCNA are of prognostic relevance in breast cancer patients.
Expression of the proteins listed above was evaluated in formalin-fixed and paraffin-embedded sections of 311 primary breast cancer specimens using modified Avidin-Biotin-Complex methods. Results were correlated to clinical and morphological parameters (age, menopausal status, nodal status, tumour size, tumour grade), and clinical course of disease (complete follow-up in 301 women, median observation time 62 months) utilising univariate and multivariate statistical analyses.
If univariate analyses and multivariate regression analyses according to the Cox-model were applied, only Cathepsin D correlated to an elevated risk for recurrence in nodally negative patients (n = 135). In nodally positive women (n = 161), increasing tumour size, tumour grade, lack of ER and PR, expression of p185neu, p53, and PCNA indicated a significantly increased relative risk.
Immunohistochemistry allows the detection of parameters which may indicate prognosis in subgroup of breast cancer patients.
我们检测了雌激素和孕激素受体（ER、PR）、雌激素依赖蛋白pS2、生长激素受体p185neu和表皮生长因子受体（EGF-R）、肿瘤抑制蛋白p53、组织蛋白酶D和尿激酶以及增殖标志物增殖细胞核抗原（PCNA）的免疫组化检测在乳腺癌患者中是否具有预后相关性。
采用改良抗生物素蛋白-生物素复合物法，对311例原发性乳腺癌标本的福尔马林固定石蜡包埋切片进行上述蛋白表达评估。利用单因素和多因素统计分析，将结果与临床和形态学参数（年龄、绝经状态、淋巴结状态、肿瘤大小、肿瘤分级）以及疾病临床进程（301名女性的完整随访，中位观察时间62个月）相关联。
如果应用单因素分析和根据Cox模型进行的多因素回归分析，仅组织蛋白酶D与淋巴结阴性患者（n = 135）复发风险升高相关。在淋巴结阳性女性（n = 161）中，肿瘤大小增加、肿瘤分级升高、缺乏ER和PR、p185neu、p53和PCNA表达表明相对风险显著增加。
免疫组化可检测出可能提示乳腺癌患者亚组预后的参数。
