Keith I M, Jin J, Saban R
Department of Comparative Biosciences, School of Veterinary Medicine, University of Wisconsin, Madison 53706-1102, USA.
J Comp Neurol. 1995 Dec 4;363(1):28-36. doi: 10.1002/cne.903630104.
Urinary bladders of normal adult female guinea pigs were analyzed for anatomical evidence of nerve-mast cell interaction using light microscopy and electron microscopy. Nerves, ganglia, and individual nerve fibers were visualized on paraffin sections using immunohistochemistry with antisera against the neural antigens neurofilament protein and protein gene product 9.5, and sections were also immunoreacted with antisera against the neuropeptides substance P and calcitonin gene-related peptide. Separate mast cell populations were identified by counterstaining with toluidine blue and alcian blue. Mast cells of both types were found within nerves and intramural ganglia and were in close contact with individual nerve fibers displaying substance P- and calcitonin gene-related peptide-like immunoreactivity. Moreover, serotonin-immunoreactive mast cells were innervated with nerve fibers that reacted with antiserum against vasoactive intestinal polypeptide. At the ultrastructural level, these fibers were almost exclusively identified as unmyelinated primary sensory afferents. Mast cells contacted these fibers with lamellipodia that wrapped around and enclosed the fibers deeply within the cell. Close association between mast cells, nerves, and vessels was common. Ultrastructural evidence suggests that bidirectional communication occurs between nerve fibers and mast cells. These structures may participate in axon reflexes that regulate normal vascular and detrusor smooth muscle function and cause vasodilation, edema, inflammation, and bladder hyperreactivity. In summary, a close relationship exists between mast cells and peptidergic nerve fibers, including primary sensory afferents. Results suggest that bidirectional interaction could occur between nerves and mast cells.
使用光学显微镜和电子显微镜对成年雌性豚鼠的膀胱进行分析，以寻找神经-肥大细胞相互作用的解剖学证据。利用针对神经抗原神经丝蛋白和蛋白基因产物9.5的抗血清，通过免疫组织化学在石蜡切片上观察神经、神经节和单个神经纤维，切片还与针对神经肽P物质和降钙素基因相关肽的抗血清进行免疫反应。通过甲苯胺蓝和阿尔辛蓝复染来识别不同的肥大细胞群体。两种类型的肥大细胞均在神经和壁内神经节中发现，并与显示P物质和降钙素基因相关肽样免疫反应性的单个神经纤维紧密接触。此外，血清素免疫反应性肥大细胞由与抗血管活性肠多肽抗血清反应的神经纤维支配。在超微结构水平上，这些纤维几乎完全被鉴定为无髓初级感觉传入纤维。肥大细胞通过片状伪足与这些纤维接触，片状伪足围绕并深入包裹细胞内的纤维。肥大细胞、神经和血管之间的紧密联系很常见。超微结构证据表明神经纤维和肥大细胞之间存在双向通讯。这些结构可能参与调节正常血管和逼尿肌平滑肌功能并导致血管舒张、水肿、炎症和膀胱高反应性的轴突反射。总之，肥大细胞与肽能神经纤维（包括初级感觉传入纤维）之间存在密切关系。结果表明神经和肥大细胞之间可能发生双向相互作用。
