主动脉中膜细胞培养物中弹性蛋白的生物合成。
The biosynthesis of elastin by an aortic medial cell culture.
作者信息
Abraham P A, Hart M L, Winge A R, Carnes W H
出版信息
Adv Exp Med Biol. 1977;79:397-412. doi: 10.1007/978-1-4684-9093-0_35.
A long term culture of aortic medial cells, derived from newborn pig aorta, has been established. The ability of these cells to synthesize soluble elastin has been demonstrated by isolation and characterization of the radioactively labeled protein, using soluble elastin of copper-deficient pig aorta as the carrier. The synthesis of corsslinked elastin was shown by the isolation and identification of the lysine-derived crosslinks after incubating the cultures with [14C]-labeled lysine. The precursor relationship of soluble to insoluble elastin was demonstrated by incubating the [3H]-labeled soluble elastin from copper-deficient pig aorta with the culture and isolating labeled crosslinks from the insoluble elastin residue.
已建立了源自新生猪主动脉的主动脉中膜细胞的长期培养体系。通过使用缺铜猪主动脉的可溶性弹性蛋白作为载体，对放射性标记蛋白进行分离和表征，证明了这些细胞合成可溶性弹性蛋白的能力。在用[¹⁴C]标记的赖氨酸孵育培养物后，通过分离和鉴定赖氨酸衍生的交联键，显示了交联弹性蛋白的合成。通过将缺铜猪主动脉的[³H]标记的可溶性弹性蛋白与培养物一起孵育，并从不溶性弹性蛋白残渣中分离出标记的交联键，证明了可溶性弹性蛋白与不溶性弹性蛋白之间的前体关系。
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