MA D, Olave I, Merino A, Reinberg D
Howard Hughes Medical Institute, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, 08854-5635, USA.
Proc Natl Acad Sci U S A. 1996 Jun 25;93(13):6583-8. doi: 10.1073/pnas.93.13.6583.
Human transcription factor IIA (TFIIA) is composed of three subunits (alpha, beta, and gamma). TFIIA interacts with the TATA-box binding protein and can overcome repression of transcription. TFIIA was found to be necessary for VP16-mediated transcriptional activation through a coactivator function. We have separated the coactivator and antirepression activities of TFIIA. A TFIIA lacking the alpha subunit was isolated from HeLa cells. This "mini-TFIIA" interacts with the TATA-box binding protein and can overcome repression of transcription, but it is defective in transcriptional coactivator function.
人类转录因子IIA（TFIIA）由三个亚基（α、β和γ）组成。TFIIA与TATA盒结合蛋白相互作用，并能克服转录抑制。通过共激活因子功能发现TFIIA对于VP16介导的转录激活是必需的。我们已经分离出TFIIA的共激活因子和抗抑制活性。从HeLa细胞中分离出一种缺乏α亚基的TFIIA。这种“微型TFIIA”与TATA盒结合蛋白相互作用，并能克服转录抑制，但在转录共激活因子功能方面存在缺陷。
Zotero 插件
