Asari Y, Majima M, Sugimoto K, Katori M, Ohwada T
Department of Pharmacology, Kitasato University School of Medicine, Kanagawa, Japan.
Shock. 1996 Mar;5(3):208-12. doi: 10.1097/00024382-199603000-00007.
Lipopolysaccharide (LPS) concentrations in the portal vein after intraperitoneal (i.p.) injection were slightly higher than those in the arteries. The tumor necrosis factor (TNF alpha) levels in arterial serum were higher after i.p. injection than after i.v. injection and rose to a peak at 90 min after some delay. Infusion of LPS into the portal vein increased the TNF alpha levels in the arterial serum. Pretreatment with indomethacin further increased the arterial levels of TNF alpha after portal infusion, but did not after them after i.p. injection, because of the reduction by indomethacin of LPS absorption after i.p. Injection of LPS. TNF alpha was also generated in the peritoneal cavity after i.p. injection of LPS. The TNF alpha concentrations in the arterial serum and in the peritoneal cavity were accelerated by mast cell degradation. In conclusion, TNF alpha was generated mainly in the liver, but also in the peritoneal cavity, after i.p. injection of LPS, and was negatively regulated by prostaglandins.
腹腔注射后门静脉中的脂多糖（LPS）浓度略高于动脉中的浓度。腹腔注射后动脉血清中的肿瘤坏死因子（TNFα）水平高于静脉注射后，且在稍有延迟后于90分钟达到峰值。向门静脉输注LPS会增加动脉血清中的TNFα水平。用吲哚美辛预处理会进一步增加门静脉输注后动脉中的TNFα水平，但腹腔注射后则不会，这是因为吲哚美辛减少了腹腔注射LPS后的LPS吸收。腹腔注射LPS后腹腔中也会产生TNFα。肥大细胞降解会加速动脉血清和腹腔中的TNFα浓度。总之，腹腔注射LPS后，TNFα主要在肝脏中产生，但也在腹腔中产生，并受到前列腺素的负调节。
