Ishii R, Shinozuka K, Kunitomo M, Hashimoto T, Takeuchi K
Department of Pharmacology, Meiji College of Pharmacy, Tokyo, Japan.
Comp Biochem Physiol C Pharmacol Toxicol Endocrinol. 1996 Mar;113(3):387-91. doi: 10.1016/0742-8413(96)00006-0.
The releases of endogenous ATP from ear, femoral, renal and pulmonary arteries of rabbit were compared. Adenyl purines, such as ATP, ADP, AMP and adenosine, and norepinephrine (NE) were quantified by HPLC-fluorescence detection and HPLC-electrochemical detection, respectively. In all four blood vessels, electrical stimulation (ES) at 16 Hz significantly increased overflow of adenyl purine and NE. ATP was the main largest among adenyl purine released; release of adenosine was very small. The rank order of amounts of total purine released by ES was ear artery > renal artery = femoral artery >> pulmonary artery. There was no significant difference among the amounts of NE release induced by ES from these four arteries. ES-induced purine release was notably reduced by denudation of endothelium and prazosin at 1 muM. Methoxamine, alpha 1-adrenoceptor agonist, also produced release of adenyl purines in the four arteries. The rank order of amount of total purine released was ear artery > renal artery = femoral artery >> pulmonary artery. These suggest that the sources of ATP released by sympathetic nerve stimulation, which seems to be alpha 1-adrenoceptors on endothelial cells, not distributed homogeneously in the vasculature.
比较了兔耳动脉、股动脉、肾动脉和肺动脉中内源性ATP的释放情况。分别通过高效液相色谱-荧光检测和高效液相色谱-电化学检测对腺苷嘌呤（如ATP、ADP、AMP和腺苷）和去甲肾上腺素（NE）进行定量分析。在所有四种血管中，16Hz的电刺激（ES）显著增加了腺苷嘌呤和NE的溢出。在释放的腺苷嘌呤中，ATP是主要的且释放量最大；腺苷的释放量非常小。ES诱导释放的总嘌呤量的排序为：耳动脉＞肾动脉 = 股动脉 >> 肺动脉。这四条动脉由ES诱导的NE释放量之间没有显著差异。内皮剥脱和1μM哌唑嗪可显著降低ES诱导的嘌呤释放。α1肾上腺素能受体激动剂甲氧明也可使四条动脉释放腺苷嘌呤。释放的总嘌呤量的排序为：耳动脉＞肾动脉 = 股动脉 >> 肺动脉。这些结果表明，交感神经刺激释放ATP的来源似乎是内皮细胞上的α1肾上腺素能受体，且在脉管系统中分布不均一。
