[人类肝细胞癌中p53突变与肿瘤侵袭性的关系]

[The relation between p53 mutations and tumor invasiveness of human hepatocellular carcinoma].

作者信息

Qin L, Tang Z, Liu K

机构信息

Liver Cancer Institute, Shanghai Medical University.

出版信息

Zhonghua Zhong Liu Za Zhi. 1995 Nov;17(6):405-8.

PMID:8697987

Abstract

We have combined the polymerase chain reaction (PCR) with the HaeIII enzyme restriction analysis to study the mutations at codon 249 of p53 gene in L02 human liver cell line, Bel-7402, SMMC-7721 human hepatocellular carcinoma cell lines and 19 surgical specimens of hepatocellular carcinoma (HCC) and their surrounding non-cancer liver tissues. None of the three cell lines and the non-cancer liver tissues was found to have p53 mutation; but 10 of 19 HCC samples (52.6%) had p53 point mutations, and the p53 gene mutated more frequently in HCC with incomplete capsule, intrahepatic metastasis, or multiple nodules than in those with well encapsulated (70% vs 33.3%, P < 0.05), no intrahepatic metastasis (71.4% vs 41.6%, P < 0.05) and single nodule (83.3% vs 38.5%, P < 0.05). These suggested that the codon 249 was a mutational hotspot of the p53 gene in human HCC seen in China, and p53 mutations may be related to invasiveness of human HCC.

摘要

我们将聚合酶链反应（PCR）与HaeIII酶切分析相结合，研究了L02人肝细胞系、Bel-7402、SMMC-7721人肝癌细胞系以及19例手术切除的肝细胞癌（HCC）标本及其癌旁非癌肝组织中p53基因第249密码子的突变情况。结果发现，三种细胞系及癌旁非癌肝组织均未检测到p53基因突变；而19例HCC标本中有10例（52.6%）存在p53点突变，且与包膜完整的HCC相比，包膜不完整、有肝内转移或多结节的HCC中p53基因突变更为频繁（70%比33.3%，P<0.05），无肝内转移者（71.4%比41.6%，P<0.05）以及单结节者（83.3%比38.5%，P<0.05）。这些结果提示，第249密码子是我国人HCC中p53基因的一个突变热点，p53基因突变可能与人HCC的侵袭性有关。