Qin L, Tang Z, Liu K
Liver Cancer Institute, Shanghai Medical University.
Zhonghua Zhong Liu Za Zhi. 1995 Nov;17(6):405-8.
We have combined the polymerase chain reaction (PCR) with the HaeIII enzyme restriction analysis to study the mutations at codon 249 of p53 gene in L02 human liver cell line, Bel-7402, SMMC-7721 human hepatocellular carcinoma cell lines and 19 surgical specimens of hepatocellular carcinoma (HCC) and their surrounding non-cancer liver tissues. None of the three cell lines and the non-cancer liver tissues was found to have p53 mutation; but 10 of 19 HCC samples (52.6%) had p53 point mutations, and the p53 gene mutated more frequently in HCC with incomplete capsule, intrahepatic metastasis, or multiple nodules than in those with well encapsulated (70% vs 33.3%, P < 0.05), no intrahepatic metastasis (71.4% vs 41.6%, P < 0.05) and single nodule (83.3% vs 38.5%, P < 0.05). These suggested that the codon 249 was a mutational hotspot of the p53 gene in human HCC seen in China, and p53 mutations may be related to invasiveness of human HCC.
我们将聚合酶链反应（PCR）与HaeIII酶切分析相结合，研究了L02人肝细胞系、Bel-7402、SMMC-7721人肝癌细胞系以及19例手术切除的肝细胞癌（HCC）标本及其癌旁非癌肝组织中p53基因第249密码子的突变情况。结果发现，三种细胞系及癌旁非癌肝组织均未检测到p53基因突变；而19例HCC标本中有10例（52.6%）存在p53点突变，且与包膜完整的HCC相比，包膜不完整、有肝内转移或多结节的HCC中p53基因突变更为频繁（70%比33.3%，P<0.05），无肝内转移者（71.4%比41.6%，P<0.05）以及单结节者（83.3%比38.5%，P<0.05）。这些结果提示，第249密码子是我国人HCC中p53基因的一个突变热点，p53基因突变可能与人HCC的侵袭性有关。
