Rotteveel R C, Roozendaal K J, Weijers R N, Eijsman L
Department of Clinical Chemistry, Onze Lieve Vrouwe Gasthuis, Amsterdam, The Netherlands.
Haemostasis. 1996 Jan-Feb;26(1):1-10. doi: 10.1159/000217181.
We investigated the anticoagulating and heparin-neutralizing properties of protamine and polybrene (hexadimethrine bromide), using the endogenous thrombin potential (ETP) as the parameter to access plasma coagulability. The hypocoagulability induced by high doses of heparin (3 IU/ml) could be reversed by addition of protamine to a very limited extent only. Polybrene on the other hand did neutralize heparin at the equivalent concentration and a two-fold excess did not influence the ETP parameters. In vivo neutralization of high-dose heparin with protamine should therefore be reconsidered. Our experiments suggest polybrene to be superior over protamine with respect to neutralization of high doses of heparin.
我们使用内源性凝血酶潜力（ETP）作为评估血浆凝固性的参数，研究了鱼精蛋白和聚凝胺（溴化六烃季铵）的抗凝和中和肝素的特性。高剂量肝素（3 IU/ml）诱导的低凝性仅在非常有限的程度上可通过添加鱼精蛋白来逆转。另一方面，聚凝胺在等效浓度下确实能中和肝素，且两倍过量的聚凝胺不会影响ETP参数。因此，应重新考虑在体内用鱼精蛋白中和高剂量肝素的做法。我们的实验表明，在中和高剂量肝素方面，聚凝胺优于鱼精蛋白。
