Donoghue A M, Byers A P, Johnston L A, Armstrong D L, Wildt D E
Department of Reproductive Physiology, National Zoological Park, Front Royal, VA 22630, USA.
J Reprod Fertil. 1996 May;107(1):53-8. doi: 10.1530/jrf.0.1070053.
The ovarian response to equine chorionic gonadotrophin (eCG) and human chorionic gonadotrophin (hCG), the effect of timing of ovulation relative to hCG injection and the use of laparoscopic intrauterine artificial insemination (AI) were examined in two subspecies of tiger (Panthera tigris). Adult female tigers were subjected to the same eCG/hCG treatment followed by laparoscopy under xylazine/diazapam/ketamine HCl anaesthesia at 39-42 h (Group I, n = 9), 46-49 h (Group II, n = 5) or 51-55 h (Group III, n = 5) after hCG. Six of these females, observed to be postovulatory at the time of laparoscopy (Group II, n = 3; Group III, n = 3), were subjected to intrauterine AI. The number of preovulatory follicles observed on the ovaries of Group I females was twofold greater (P < 0.05) than the number observed on ovaries of females in Group II and III. Fewer (P < 0.05) corpora lutea were observed on ovaries of Group I females (1.3 +/- 0.6) compared with the number of corpora lutea in Group II and III (combined average, 7.8 +/- 0.8 corpora lutea per female). Only one of ten females in Groups II and III failed to ovulate by the time of laparoscopy. Four Group I females never ovulated, based on a laparoscopic re-evaluation 4 weeks later. One female inseminated 46 h after hCG (Group II) became pregnant and delivered a healthy cub after a normal gestation. There were no apparent differences between subspecies in response to the same ovulation induction protocol. Results demonstrate the importance of the relationship between exogenous gonadotrophin treatment and onset of anaesthesia for laparoscopic examination and AI in tigers. Data clearly indicate that anaesthesia/laparoscopy conducted too early (39-42 h after hCG) compromises the number of females and proportion of follicles ovulating. In contrast, ovulation success is high if anaesthesia/laparoscopy is performed after this time, and intrauterine insemination can result in healthy young.
在两种老虎亚种（ Panthera tigris ）中，研究了卵巢对马绒毛膜促性腺激素（eCG）和人绒毛膜促性腺激素（hCG）的反应、排卵时间相对于hCG注射时间的影响以及腹腔镜子宫内人工授精（AI）的应用。成年雌性老虎接受相同的eCG/hCG处理，然后在注射hCG后39 - 42小时（I组，n = 9）、46 - 49小时（II组，n = 5）或51 - 55小时（III组，n = 5），在赛拉嗪/地西泮/氯胺酮盐酸盐麻醉下进行腹腔镜检查。其中6只在腹腔镜检查时被观察到已排卵的雌性（II组，n = 3；III组，n = 3）接受了子宫内人工授精。I组雌性卵巢上观察到的排卵前卵泡数量比II组和III组雌性卵巢上观察到的数量多两倍（P < 0.05）。与II组和III组相比，I组雌性卵巢上观察到的黄体数量更少（P < 0.05）（I组为1.3 ± 0.6个，II组和III组合并平均每只雌性为7.8 ± 0.8个黄体）。II组和III组的十只雌性中只有一只在腹腔镜检查时未排卵。根据4周后腹腔镜复查，I组有4只雌性从未排卵。一只在hCG后46小时授精的雌性（II组）怀孕，并在正常妊娠期后产下一只健康的幼崽。在对相同排卵诱导方案的反应中，两个亚种之间没有明显差异。结果表明，在老虎中，外源性促性腺激素处理与腹腔镜检查和人工授精麻醉开始之间的关系很重要。数据清楚地表明，过早（hCG后39 - 42小时）进行麻醉/腹腔镜检查会影响排卵的雌性数量和卵泡比例。相比之下，如果在此时间之后进行麻醉/腹腔镜检查，排卵成功率很高，并且子宫内授精可以生出健康的幼崽。
