Sato Y, Asada Y, Marutsuka K, Hatakeyama K, Sumiyoshi A
First Department of Pathology, Miyazoki Medical College, Japan.
Thromb Haemost. 1996 Mar;75(3):389-92.
Tissue factor (TF) plays a key role as a primary initiator on the extrinsic coagulation cascade. Recently, upregulation of TF has been reported in human atherosclerotic lesions. We investigated the effects of TF on migration and proliferation of cultured smooth muscle cells (SMCs) from rabbit aortas. We tested three kinds of recombinant human TF (L-TF: the full length of TF with relipidation, NL-TF: the full length of TF without relipidation, and S-TF: a soluble form of TF1-219). Only L-TF had coagulant activity. All kinds of TF showed the chemotactic migration activity for SMCs. The migration ability of TFs was comparable to those of platelet-derived growth factor (PDGF)-BB and basic fibroblast-growth factor (bFGF), and was inhibited by anti-TF polyclonal and monoclonal antibodies. On the other hand, none of the forms of TF induced SMC proliferation. These results indicate that TF is not only a coagulation factor but also a strong chemotactic factor for vascular SMCs, and suggest that TF could play an important role in atherogenesis as well as in hemostasis and thrombosis.
组织因子（TF）作为外源性凝血级联反应的主要启动因子发挥关键作用。最近，有报道称TF在人类动脉粥样硬化病变中上调。我们研究了TF对兔主动脉培养的平滑肌细胞（SMC）迁移和增殖的影响。我们测试了三种重组人TF（L-TF：经脂质化处理的全长TF，NL-TF：未经脂质化处理的全长TF，以及S-TF：TF1-219的可溶性形式）。只有L-TF具有凝血活性。所有类型的TF均对SMC表现出趋化迁移活性。TFs的迁移能力与血小板衍生生长因子（PDGF）-BB和碱性成纤维细胞生长因子（bFGF）相当，且被抗TF多克隆抗体和单克隆抗体抑制。另一方面，所有形式的TF均未诱导SMC增殖。这些结果表明，TF不仅是一种凝血因子，也是血管SMC的一种强趋化因子，并提示TF可能在动脉粥样硬化形成以及止血和血栓形成中发挥重要作用。
