一种具有抗白细胞介素-1β转化酶活性的黏液瘤病毒编码的丝氨酸蛋白酶抑制剂样蛋白的特性分析。
Characterization of a myxoma virus-encoded serpin-like protein with activity against interleukin-1 beta-converting enzyme.
作者信息
Petit F, Bertagnoli S, Gelfi J, Fassy F, Boucraut-Baralon C, Milon A
机构信息
Laboratoire Associé de Microbiologie, Moléculaire, Institut National de la Recherche Agronomique, Toulouse, France.
出版信息
J Virol. 1996 Sep;70(9):5860-6. doi: 10.1128/JVI.70.9.5860-5866.1996.
Abstract
A genomic library of myxoma virus (MV) DNA, a leporipoxvirus that causes myxomatosis, was constructed and screened by in vitro transcription-translation. A clone was selected on the basis of its strong reactivity with MV antiserum. Analysis of the corresponding DNA sequence and the deduced amino acid sequence revealed an open reading frame coding for a 34-kDa protein with strong homologies to members of the serpin superfamily. The gene encoding this new protein, called serp2, was localized on the MV genome. Interestingly, this gene is deleted in an attenuated strain. We constructed a baculovirus vector to produce recombinant Serp2 protein and raised specific antisera that allowed the characterization of Serp2 expression during the MV cycle. The biological relevance of this new serpin from MV was monitored, and it was shown that Serp2 could inhibit human interleukin-1 beta-converting enzyme activity.
摘要
构建了黏液瘤病毒（MV）DNA的基因组文库，黏液瘤病毒是一种引起黏液瘤病的兔痘病毒，并通过体外转录翻译进行筛选。基于其与MV抗血清的强反应性选择了一个克隆。对相应DNA序列和推导的氨基酸序列的分析揭示了一个开放阅读框，其编码一种与丝氨酸蛋白酶抑制剂超家族成员具有高度同源性的34 kDa蛋白。编码这种新蛋白（称为Serp2）的基因定位于MV基因组上。有趣的是，该基因在一个减毒株中缺失。我们构建了杆状病毒载体来生产重组Serp2蛋白，并制备了特异性抗血清，这使得能够对MV周期中Serp2的表达进行表征。监测了这种来自MV的新丝氨酸蛋白酶抑制剂的生物学相关性，结果表明Serp2可以抑制人白细胞介素-1β转换酶的活性。
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Characterization of a myxoma virus-encoded serpin-like protein with activity against interleukin-1 beta-converting enzyme.一种具有抗白细胞介素-1β转化酶活性的黏液瘤病毒编码的丝氨酸蛋白酶抑制剂样蛋白的特性分析。
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