Silveira R M, Fiorentin L, Marques E K
Departamento de Bioquímica, UFRGS Porto Alegre, Rio Grande do Sol, Brazil.
Avian Dis. 1996 Jan-Mar;40(1):218-22.
Mycoplasma gallisepticum- or M. synoviae-challenged chickens were monitored with serological assays (serum plate agglutination, hemagglutination inhibition, and enzyme-linked immunosorbent assay) and polymerase chain reaction (PCR). The tracheal swabs from M. gallisepticum-challenged chickens received three different treatments (phosphate-buffered saline [PBS], Frey's broth, or 10 mM Tris-HCl/250 mM ethylenediaminetetraacetic acid/ 2.5% sodium dodecyl sulfate [STE]) prior to DNA purification. A nonphenolic method for DNA extraction was utilized. The best PCR results were obtained with PBS swab treatment. The nonphenolic method for DNA extraction was compared with a phenolic method in an experiment with tracheal swabs from M. synoviae-challenged chickens and commercial flocks. Both methods gave comparable results.
对感染鸡败血支原体或滑液支原体的鸡进行血清学检测（血清平板凝集试验、血凝抑制试验和酶联免疫吸附测定）及聚合酶链反应（PCR）监测。在进行DNA纯化之前，对感染鸡败血支原体的鸡的气管拭子进行了三种不同处理（磷酸盐缓冲盐水[PBS]、弗雷氏肉汤或10 mM三羟甲基氨基甲烷盐酸盐/250 mM乙二胺四乙酸/2.5%十二烷基硫酸钠[STE]）。采用了一种非酚法进行DNA提取。采用PBS拭子处理获得了最佳PCR结果。在一项针对感染滑液支原体的鸡的气管拭子和商业鸡群的实验中，将非酚法DNA提取与酚法进行了比较。两种方法得到的结果相当。
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