Einspanier A, Ivell R, Hodges J K
Department of Reproduction, German Primate Center, Göttingen, Germany.
Adv Exp Med Biol. 1995;395:517-22.
The distributions of oxytocin (OT) and the oxytocin receptor (OTR) have been characterised in preovulatory follicles of the marmoset monkey and are described in relation to the process of luteinisation using a combination of in vitro and in vivo techniques, including immunohistochemistry and granulosa cell culture. Ovaries were collected in the periovulatory phase before and 22h after exogenous gonadotropin (hCG) treatment, but prior to ovulation. Before hCG treatment, OT immunoreactivity (OT-ir) was found mostly in granulosa cells of antral follicles, especially in the layers nearest the antrum. In contrast, oxytocin receptor immunoreactivity (OTR-ir) was observed principally in basal granulosa cells of antral follicles. Progesterone receptor (PR) and 3 beta hydroxysteroid dehydrogenase (3 beta HSD), markers for luteinisation, were negative for all ovarian tissues collected before hCG treatment. After hCG treatment, almost all granulosa cell layers in antral follicles stained positively for both OT-ir and OTR-ir, most prominently in preovulatory follicles and especially in the cumulus oophorus. This increase in staining was associated with an induction of PR and 3 beta HSD activity in the granulosa cells of preovulatory follicles. OT production by granulosa cells in culture was stimulated by the addition of hCG, but only in cultures derived from preovulatory follicles; whereas FSH was without any effect. Addition of extrinsic OT to the cultures elicited an increase in progesterone production only for the granulosa cells derived from preovulatory follicles. Together, the results of the in vivo and in vitro studies point to an involvement of OT in the process of leuteinisation in the marmoset monkey.
已对狨猴排卵前卵泡中催产素（OT）和催产素受体（OTR）的分布进行了表征，并结合体外和体内技术（包括免疫组织化学和颗粒细胞培养），描述了它们与黄体化过程的关系。在促性腺激素（hCG）治疗前及治疗后22小时但排卵前收集卵巢。在hCG治疗前，OT免疫反应性（OT-ir）主要见于窦状卵泡的颗粒细胞，尤其是最靠近卵泡腔的层。相比之下，催产素受体免疫反应性（OTR-ir）主要见于窦状卵泡的基底颗粒细胞。黄体化标志物孕酮受体（PR）和3β-羟基类固醇脱氢酶（3β-HSD）在hCG治疗前收集的所有卵巢组织中均为阴性。hCG治疗后，窦状卵泡中的几乎所有颗粒细胞层对OT-ir和OTR-ir均呈阳性染色，在排卵前卵泡中最为明显，尤其是在卵丘中。这种染色增加与排卵前卵泡颗粒细胞中PR和3β-HSD活性的诱导有关。在培养物中添加hCG可刺激颗粒细胞产生OT，但仅在源自排卵前卵泡的培养物中；而FSH无任何作用。向培养物中添加外源性OT仅能使源自排卵前卵泡的颗粒细胞的孕酮分泌增加。体内和体外研究结果共同表明，OT参与了狨猴的黄体化过程。
