Maslak D M, Reynolds D L
Department of Microbiology, Immunology, and Preventive Medicine, Iowa State University, Ames 50011, USA.
Avian Dis. 1995 Oct-Dec;39(4):736-42.
The head-associated lymphoid tissues of the chicken, composed of the harderian gland and the conjunctiva-associated lymphoid tissue (CALT), were studied to determine whether changes occurred in lymphocyte subpopulations as chickens age from 1 week to 8 weeks. The B cells and subpopulations of T-lymphocytes of the head-associated lymphoid tissues were identified using in situ immunohistochemical staining. Monoclonal antibodies specific for various lymphocyte surface antigens were used. The concentration of T-lymphocytes, particularly CD3+ and CD4+ cells, within the harderian gland increased with age, whereas the concentration of B cells remained the same. B-lymphocytes were observed within the germinal centers of the CALT of 4-week-old birds. The T-lymphocytes within the CALT surrounded the B-cell-rich germinal centers. CD3+ T-lymphocytes were the predominant cell population in all age groups examined. Increasing concentrations of CD3+, CD4+, and CD8+ T-lymphocytes were observed within the CALT as chicks developed from 1 week to 4 weeks of age. Finally, no changes were observed in lymphocyte populations within the CALT as chicks developed from 4 weeks to 8 weeks of age.
对鸡的头部相关淋巴组织进行了研究，该组织由哈德氏腺和结膜相关淋巴组织（CALT）组成，目的是确定随着鸡从1周龄到8周龄的生长，淋巴细胞亚群是否会发生变化。使用原位免疫组织化学染色法鉴定头部相关淋巴组织中的B细胞和T淋巴细胞亚群。使用了针对各种淋巴细胞表面抗原的单克隆抗体。哈德氏腺内T淋巴细胞，特别是CD3 +和CD4 +细胞的浓度随年龄增长而增加，而B细胞的浓度保持不变。在4周龄鸡的CALT生发中心内观察到B淋巴细胞。CALT内的T淋巴细胞围绕着富含B细胞的生发中心。在所有检查的年龄组中，CD3 + T淋巴细胞是主要的细胞群体。随着雏鸡从1周龄发育到4周龄，在CALT内观察到CD3 +、CD4 +和CD8 + T淋巴细胞的浓度增加。最后，随着雏鸡从4周龄发育到8周龄，未观察到CALT内淋巴细胞群体的变化。
