Kearsey S E, Labib K, Maiorano D
Department of Zoology, University of Oxford, UK.
Curr Opin Genet Dev. 1996 Apr;6(2):208-14. doi: 10.1016/s0959-437x(96)80052-9.
A clearer picture of replication control is emerging through the characterization of proteins, such as cdc18/Cdc6 and members of the mini-chromosome maintenance (MCM) protein family, that are involved in the initiation step. Cyclin B dependent kinases have conserved roles in both Saccharomyces cerevisiae and Schizosaccharomyces pombe, switching on DNA replication in G1 and preventing re-replication in G2. A model is suggested where MCMs and CDKs play complementary roles to ensure 'once-per-cell-cycle' replication, with CDKs maintaining a G1 or G2 state, whereas MCMs provide a cis-acting control on chromatin to prevent reinitiation during a single S phase.
通过对参与起始步骤的蛋白质（如cdc18/Cdc6和微型染色体维持（MCM）蛋白家族成员）的表征，复制控制的图景正变得更加清晰。细胞周期蛋白B依赖性激酶在酿酒酵母和粟酒裂殖酵母中都具有保守作用，在G1期开启DNA复制并防止G2期重新复制。有人提出了一个模型，其中MCM和CDK发挥互补作用以确保“每个细胞周期一次”的复制，CDK维持G1或G2状态，而MCM对染色质提供顺式作用控制以防止在单个S期重新起始。
