多瘤病毒主要衣壳蛋白VP1的甲基化

Methylation of the polyomavirus major capsid protein VP1.

作者信息

Burton K S, Consigli R A

机构信息

Division of Biology, Kansas State University, Manhattan 66506, USA.

出版信息

Virus Res. 1996 Feb;40(2):141-7. doi: 10.1016/0168-1702(95)01266-4.

DOI:10.1016/0168-1702(95)01266-4

PMID:8725110

Abstract

Polyomavirus VP1 has been shown to be modified by phosphorylation, sulfation, acetylation and hydroxylation. The major capsid protein VP1 is now shown to be modified by methylation. Addition of cycloheximide to infected cultures followed by addition of [3H-methyl]-L-methionine and subsequent immunoprecipitation, SDS-PAGE and fluorography revealed methylation occurring on VP1. Amino acid analysis of [3H-methyl]-L-methionine-labelled polyomavirus VP1 by two-dimensional paper chromatography and HPLC of the acid-hydrolyzed protein revealed the presence of 3H-labelled trimethyllysine and monomethylarginine.

摘要

多瘤病毒VP1已被证明可通过磷酸化、硫酸化、乙酰化和羟基化进行修饰。现在发现主要衣壳蛋白VP1可被甲基化修饰。向感染的培养物中加入环己酰亚胺，随后加入[3H-甲基]-L-甲硫氨酸，接着进行免疫沉淀、SDS-PAGE和荧光自显影，结果显示VP1上发生了甲基化。通过二维纸层析和酸水解蛋白的HPLC对[3H-甲基]-L-甲硫氨酸标记的多瘤病毒VP1进行氨基酸分析，结果显示存在3H标记的三甲基赖氨酸和一甲基精氨酸。