Monge R, Arias M L, Antillón F, Utzinger D
Facultad de Microbiología, Universidad de Costa Rica.
Arch Latinoam Nutr. 1995 Jun;45(2):117-21.
The sanitary quality of street sold fruits was analyzed during the period from march 1990 thru march 1993 in San Jose, Costa Rica. It looked for the presence of Salmonella spp. Shigella spp., Escherichia coli as well as fecal coliforms in natural refreshments, fruit salads and the fruits most frecuently expended on streets, either in slices as the pineapple (Ananas comosus), papaya (Carica papaya), non-ripe mangoe (Mangifera indica) and watermelon (Citrullus vulgaris) and those that can be eaten without peeling, like nances (Byrsonima crassifolia) and jocotes (Spondias purpurea). 25 samples of each fruit, 50 natural refreshments and 50 fruit salads were processed according to rinse solution method, and the bacteriological determination was based in the methodology described by Vanderzant & Splittstoesser and the Bacteriological Analytical Manual. In the same way, it was used the Most Probable Number for 5 tubes described in the Standar Methods of Water and Wastewater in orden to analyze 15 samples of ready to use water by the fruit hawker. The nutritional value was studied according to the food composition tables for Costa Rica, Latin America and USA. The results show that more than 30% of fruit samples, 70% of natural refreshments and 96% of fruit salad presented fecal coliforms. Same time, all of them present important contamination indexes with E. coli. Salmonella spp. and Shigella spp. were not isolated. The water analysis revelead that 53% contained fecal coliforms, probably due to the lack of hygiene in the utensils used to collect water. The nutritional evaluation shows that fruit portions (except watermelon) satisfy more than 100% of the diary recommendation of vitamin C (60 mg) and 4-7% of the recommended ingestion of dietetic fiber (30g).
1990年3月至1993年3月期间,对哥斯达黎加圣何塞街头售卖的水果的卫生质量进行了分析。研究了天然饮品、水果沙拉以及街头最常消费的水果中沙门氏菌属、志贺氏菌属、大肠杆菌以及粪大肠菌群的存在情况,这些水果包括切片的菠萝(凤梨)、木瓜、未成熟芒果和西瓜,以及无需去皮即可食用的水果,如南茜果和乔科特果。按照冲洗溶液法对每种水果的25个样本、50份天然饮品和50份水果沙拉进行处理,细菌学测定基于范德赞特和斯普利茨托塞尔以及《细菌学分析手册》中描述的方法。同样,使用《水和废水标准方法》中描述的5管最可能数法对水果小贩准备使用的15份水样进行分析。根据哥斯达黎加、拉丁美洲和美国的食物成分表研究了营养价值。结果表明,超过30%的水果样本、70%的天然饮品和96%的水果沙拉中存在粪大肠菌群。同时,所有样本的大肠杆菌污染指数都很高。未分离出沙门氏菌属和志贺氏菌属。水分析显示,53%的水样含有粪大肠菌群,这可能是由于取水用具缺乏卫生所致。营养评估表明,水果部分(西瓜除外)满足了每日维生素C推荐摄入量(60毫克)的100%以上,以及膳食纤维推荐摄入量(30克)的4 - 7%。