Alvarez F, Flaño E, Castillo A, López-Fierro P, Razquin B, Villena A
Departamento de Biología Celular y Anatomía, Facultad de Biología, Universidad de León, Spain.
Anat Rec. 1996 May;245(1):17-24. doi: 10.1002/(SICI)1097-0185(199605)245:1<17::AID-AR4>3.0.CO;2-C.
Barrier cells have been recognized as a discrete group of fibroblastic- or myofibroblastic-like cells located in the lymphoid and hematopoietic organs of mammals. This paper reports the results of a morphological study of the main lymphoid organs of three salmonid species, in which cells structurally similar to the mammalian barrier cells were observed in healthy animals.
The spleen, kidney, and thymus of fingerlings of rainbow trout, Oncorhynchus mykiss, and Coho salmon, Oncorhynchus kisutch, and of adult brown trout, Salmo trutta fario, were processed for electron microscopy study using various fixation methods. Semithin sections were used for the Periodic Acid-Schiff (PAS) staining technique, and for the demonstration of the endogenous peroxidase activity.
The kidney and spleen of all the species contained a variable, but usually low, number of electron-dense, elongated, and branched cells, ultrastructurally similar to the mammalian barrier cells. They also occurred in the thymus of some brown trout and Coho salmon, but not of rainbow trout. The electron density of this cell type was present after the various types of fixation procedures. They show numerous ribosomes, well-developed secretory organelles, electron-clear vesicles, large granules, and microfilaments. In all the salmonid species, barriers cells were positive for PAS staining and for endogenous peroxidase activity. A small number of barrier cells were in mitosis. In the different organs barrier cells appeared as isolated cells, or forming syncytial networks. They were found lining the blood sinusoids of the splenic red pulp and of the renal hematopoietic tissue, in the periellipsoidal sheaths, and closely associated with erythropoietic and plasmacytopoietic foci.
Our results demonstrate the presence of cells closely resembling mammalian barrier cells in the hematopoietic and lymphoid organs of salmonids. The structure and tissue distribution of the salmonid barrier cells are discussed in relation to the functional roles described for this cell type in mammals.
屏障细胞已被确认为位于哺乳动物淋巴和造血器官中的一组离散的成纤维细胞样或肌成纤维细胞样细胞。本文报道了对三种鲑科鱼类主要淋巴器官的形态学研究结果，在健康动物中观察到了结构上与哺乳动物屏障细胞相似的细胞。
对虹鳟（Oncorhynchus mykiss）、银大麻哈鱼（Oncorhynchus kisutch）幼鱼以及成年褐鳟（Salmo trutta fario）的脾脏、肾脏和胸腺进行处理，采用各种固定方法进行电子显微镜研究。半薄切片用于过碘酸希夫（PAS）染色技术以及内源性过氧化物酶活性的显示。
所有物种的肾脏和脾脏中都含有数量不等但通常较少的电子致密、细长且有分支的细胞，超微结构上与哺乳动物屏障细胞相似。它们也存在于一些褐鳟和银大麻哈鱼的胸腺中，但虹鳟的胸腺中没有。这种细胞类型的电子密度在各种固定程序后都存在。它们显示出大量核糖体、发达的分泌细胞器、电子透明的囊泡、大颗粒和微丝。在所有鲑科鱼类中，屏障细胞对PAS染色和内源性过氧化物酶活性呈阳性。少数屏障细胞处于有丝分裂状态。在不同器官中，屏障细胞表现为单个细胞或形成合胞体网络。它们被发现位于脾红髓和肾造血组织的血窦内衬、椭球周围鞘中，并与红细胞生成灶和浆细胞生成灶密切相关。
我们的结果表明，在鲑科鱼类的造血和淋巴器官中存在与哺乳动物屏障细胞非常相似的细胞。结合哺乳动物中描述的这种细胞类型的功能作用，讨论了鲑科鱼类屏障细胞的结构和组织分布。
