[结直肠腺瘤及癌组织中P21、P53、P185蛋白表达及ras、p53基因变异的检测]

[Detection of the expression of P21, P53, P185 proteins and the mutation of ras, p53 genes in colorectal adenoma and carcinoma].

作者信息

Liang Z, Liu F, Luo Y

机构信息

Department of Pathology, Southwest Hospital, Third Military Medical University, Chongqing.

出版信息

Zhonghua Bing Li Xue Za Zhi. 1995 Dec;24(6):352-5.

PMID:8732090

Abstract

Expression of P21, P53, P185 proteins, mutations of ras, p53 genes in colorectal adenoma, carcinoma and transitional mucosa were studied using immunohistochemicstry and PCR-RFLP methods. The results showed that the positive rates of P21, P53 and P185 proteins in colorectal adenoma were 53.3%, 27.6% and 13.3% respectively, the expression of P21 and P53 were associated with the malignant potential of adenoma. The positive rates of P21, P53 and P185 proteins in colorectal carcinoma were 72.9%, 37.8% and 47.2% respectively. 9 adenomas and 40 carcinomas contained more than two protein expressions and their co-expression was associated with the malignant potential of adenoma and the prognosis of carcinoma. The mutation rates of ras gene in colorectal adenoma and carcinoma were 26.7% and 41.9% respectively. The ras gene mutation was associated with the malignant potential of adenoma. The mutation rates of p53 gene (codon 248) in adenoma and carcinoma were 3.3% and 14.9% respectively. The prognosis of patients having gene mutation of both ras and p53 were poor. The results suggested that the alterations of ras, p53 and c-erbB-2 genes are involved in the tumorigenesis and development of colorectal carcinoma.

摘要

采用免疫组织化学和聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法，研究了P21、P53、P185蛋白的表达以及ras、p53基因在大肠腺瘤、癌和移行黏膜中的突变情况。结果显示，大肠腺瘤中P21、P53和P185蛋白的阳性率分别为53.3%、27.6%和13.3%，P21和P53的表达与腺瘤的恶性潜能相关。大肠癌中P21、P53和P185蛋白的阳性率分别为72.9%、37.8%和47.2%。9例腺瘤和40例癌含有两种以上蛋白表达，它们的共表达与腺瘤的恶性潜能和癌的预后相关。大肠腺瘤和癌中ras基因的突变率分别为26.7%和41.9%。ras基因突变与腺瘤的恶性潜能相关。腺瘤和癌中p53基因（密码子248）的突变率分别为3.3%和14.9%。ras和p53基因均突变的患者预后较差。结果提示，ras、p53和c-erbB-2基因的改变参与了大肠癌的发生和发展。