Michael G J, Priestley J V
Division of Physiology, UMDS St Thomas's Hospital Medical School Campus, London, UK.
Neuroreport. 1995 Dec 29;7(1):338-42.
In situ hybridization combined with intraperitoneal injection of the tracer Fluoro-Gold was used to study the distribution of GAP-43 mRNA in autonomic cell groups of the rat spinal cord. In the thoracic cord, numerous cells expressing GAP-43 mRNA were present in the central autonomic nucleus (lamina X) and in the intermediolateral cell column (IML). In the IML, all Fluoro-Gold-labelled preganglionic neurones were heavily labelled for GAP-43 mRNA. In contrast, Fluoro-Gold-labelled motoneurones were unlabelled or only lightly labelled. Our results indicate that preganglionic sympathetic neurones express GAP-43, a conclusion that is consistent with studies showing that GAP-43 immunoreactive terminals are present in autonomic ganglia. GAP-43 in these neurones may play a role in their continuing anatomical remodelling.
采用原位杂交结合向腹腔注射示踪剂荧光金的方法，研究大鼠脊髓自主神经细胞群中GAP - 43 mRNA的分布。在胸段脊髓，中央自主神经核（X层）和中间外侧细胞柱（IML）中有大量表达GAP - 43 mRNA的细胞。在IML中，所有荧光金标记的节前神经元均被GAP - 43 mRNA大量标记。相比之下，荧光金标记的运动神经元未被标记或仅被轻度标记。我们的结果表明，节前交感神经元表达GAP - 43，这一结论与显示自主神经节中存在GAP - 43免疫反应性终末的研究一致。这些神经元中的GAP - 43可能在其持续的解剖重塑中发挥作用。
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