根癌土壤杆菌virG基因酸性pH诱导型P2启动子的切除与诱变
Resection and mutagenesis of the acid pH-inducible P2 promoter of the Agrobacterium tumefaciens virG gene.
作者信息
Chang C H, Winans S C
机构信息
Section of Microbiology, Cornell University, Ithaca, New York 14853, USA.
出版信息
J Bacteriol. 1996 Aug;178(15):4717-20. doi: 10.1128/jb.178.15.4717-4720.1996.
Abstract
Transcription of the virG gene initiates from two tandem promoters, designated P1 and P2, that are located 50 nucleotides apart. Transcription of the P2 promoter is induced by extracellular acidity. cis-acting sites required for P2 activity were identified by constructing and assaying a series of 5' and 3' resections and site-directed nucleotide substitutions. Nucleotides between positions -9 and -37 were sufficient for regulated promoter activity. Within this region, nucleotide substitutions at the predicted -10 and -35 regions strongly reduced P2 expression. In addition, alterations in the region between nucleotides -24 and -32 also eliminated or strongly reduced promoter activity. These data suggest that this promoter may be regulated by a positive transcription factor that binds to nucleotide residues in this interval.
摘要
virG基因的转录起始于两个串联启动子，分别命名为P1和P2，它们相距50个核苷酸。P2启动子的转录受细胞外酸性环境诱导。通过构建和检测一系列5'端和3'端切除片段以及定点核苷酸替换，确定了P2活性所需的顺式作用位点。-9至-37位之间的核苷酸对于启动子活性的调控是足够的。在该区域内，预测的-10和-35区域的核苷酸替换极大地降低了P2的表达。此外，-24至-32位核苷酸之间区域的改变也消除或极大地降低了启动子活性。这些数据表明，该启动子可能受一个与该区间核苷酸残基结合的正转录因子调控。
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本文引用的文献
1
Virulence genes A, G, and D mediate the double-stranded border cleavage of T-DNA from the Agrobacterium Ti plasmid.
Proc Natl Acad Sci U S A. 1987 Apr;84(7):1881-5. doi: 10.1073/pnas.84.7.1881.
2
A plant cell factor induces Agrobacterium tumefaciens vir gene expression.
Proc Natl Acad Sci U S A. 1986 Jan;83(2):379-83. doi: 10.1073/pnas.83.2.379.
3
Protein-protein communication within the transcription apparatus.
J Bacteriol. 1993 May;175(9):2483-9. doi: 10.1128/jb.175.9.2483-2489.1993.
4
The chromosomal response regulatory gene chvI of Agrobacterium tumefaciens complements an Escherichia coli phoB mutation and is required for virulence.
J Bacteriol. 1993 Oct;175(20):6626-36. doi: 10.1128/jb.175.20.6626-6636.1993.
5
A chromosomally encoded two-component sensory transduction system is required for virulence of Agrobacterium tumefaciens.
J Bacteriol. 1993 Oct;175(20):6614-25. doi: 10.1128/jb.175.20.6614-6625.1993.
6
Influence of pH on bacterial gene expression.
Mol Microbiol. 1993 Apr;8(1):5-14. doi: 10.1111/j.1365-2958.1993.tb01198.x.
7
Nucleotide sequence of the adi gene, which encodes the biodegradative acid-induced arginine decarboxylase of Escherichia coli.
J Bacteriol. 1993 Mar;175(5):1221-34. doi: 10.1128/jb.175.5.1221-1234.1993.
8
The minus 35-recognition region of Escherichia coli sigma 70 is inessential for initiation of transcription at an "extended minus 10" promoter.
J Mol Biol. 1993 Jul 20;232(2):406-18. doi: 10.1006/jmbi.1993.1400.
9
A third recognition element in bacterial promoters: DNA binding by the alpha subunit of RNA polymerase.
Science. 1993 Nov 26;262(5138):1407-13. doi: 10.1126/science.8248780.
10
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