Allen L J, Kabbur M B, Cullor J S, Gardner I A, Stott J L, George L W
Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis 95616, USA.
J Am Vet Med Assoc. 1996 Aug 1;209(3):638-42.
To evaluate alterations in lymphocyte subpopulations, CBC results, and clinical signs in neonatal calves inoculated with 3 commercially available proprietary multiple-antigen vaccines containing known quantities of endotoxin.
Prospective, randomized controlled field trial.
36 healthy Holstein heifer calves between 3 and 31 days old.
Vaccines were administered to 18 calves according to label instructions, except for the recommended age of administration. The 18 other calves served as unvaccinated controls. Two weeks after entry into the study, calves were given secondary doses of the same vaccines. Calves in both groups were examined and blood samples were collected for determination of lymphocyte subpopulations and hematological parameters once daily for 5 days beginning on the day that both the primary and the secondary vaccinations were given. Lymphocyte subpopulations, including BoCD2+, BoCD4+, BoCD8+, B cells, and gamma/delta T cells, were determined by use of flow cytometry, using monoclonal antibodies as markers.
Vaccinated calves did not develop clinical signs of illness. There were no significant differences in absolute numbers of lymphocyte subpopulations between vaccinated and unvaccinated calves. Vaccinated calves had significantly higher rectal temperatures, total WBC counts, and absolute neutrophil counts than did control calves. These differences persisted for 3 to 4 days after vaccination.
Findings confirm empirical observations that vaccination with multiple products at the same time may induce evidence of an inflammatory response in most calves. Additional research is indicated to further evaluate the safety of using multiple vaccines simultaneously.
评估接种3种市售含已知内毒素量的多抗原专利疫苗的新生犊牛淋巴细胞亚群、全血细胞计数结果及临床体征的变化。
前瞻性、随机对照现场试验。
36头3至31日龄健康的荷斯坦小母牛犊。
18头犊牛按照标签说明接种疫苗，但接种年龄除外。另外18头犊牛作为未接种疫苗的对照。进入研究两周后，给犊牛接种相同疫苗的第二剂。两组犊牛均接受检查，并在首次和第二次接种疫苗当天开始的5天内每天采集血样，用于测定淋巴细胞亚群和血液学参数。使用单克隆抗体作为标记物，通过流式细胞术测定淋巴细胞亚群，包括BoCD2+、BoCD4+、BoCD8+、B细胞和γ/δT细胞。
接种疫苗的犊牛未出现疾病临床体征。接种疫苗和未接种疫苗的犊牛淋巴细胞亚群绝对数量无显著差异。接种疫苗的犊牛直肠温度、白细胞总数和中性粒细胞绝对计数显著高于对照犊牛。这些差异在接种疫苗后持续3至4天。
研究结果证实了经验性观察结果，即同时接种多种产品疫苗可能会在大多数犊牛中引发炎症反应迹象。需要进一步研究以进一步评估同时使用多种疫苗的安全性。
