Beasley D G, Beard J, Stanfield J W, Roberts L K
Schering-Plough HealthCare Products, Memphis, TN 38151, USA.
Photochem Photobiol. 1996 Aug;64(2):303-9. doi: 10.1111/j.1751-1097.1996.tb02462.x.
Expense and inconvenience have restricted the use of the filtered xenon are lamp (solar simulator) as a UV source for conducting large-scale animal studies. Because sunscreen immunoprotective levels are significantly affected by the UV power spectrum of the source it is imperative that a solar simulating source be used for accurate measurements of sunscreen protection levels that are relevant to human LV exposures from sunlight. However, relatively inexpensive sunlamps, e.g. the UVA-340, that emit a UV power spectrum similar to that of a solar simulator are available. Unlike FS-type UVB sunlamps, which have a significant amount of effective immunosuppressive non-solar UV energy at wavelengths below 295 nm, the immunosuppression effectiveness spectrum of UVA-340 sunlamps was nearly identical to that of a solar simulator. The purpose of this study was to evaluate this sunlamp for conducting photoimmunological and sunscreen immune protection studies. Groups of C3H mice were exposed to a range of UVA-340 sunlamp doses (0.25 kJ/m2 to 20.0 kJ/m2) to establish a dose-response curve and determine the minimum immune suppression dose (MISD) for iduction of local-type suppression of contact hypersensitivity (CH). The MISD, defined as the lowest UV dose given to produce approximately 50% suppression of the CH response in mice, was determined to be 1.0 kJ/m2 for UVA-340 sunlamps. Immune protection tests on four marketed sunscreen lotions (sun protection factors [SPF] 4, 8, 15 and 30) were then conducted with UVA-340 sunlamps using MISD as the endpoint. The immune protection factors for these sunscreens were equivalent to the level of protection predicted by their labeled SPF. These results are similar to those we have previously obtained using a solar simulator. We conclude from these data that the immunosuppressive effects of UVA-340 sunlamps are similar to those of a solar simulator; however, further studies are needed to determine if UVA-340, or similar, sunlamps are a viable alternative to the solar simulator for conducting large-scale animal experiments that require a relevant UV solar spectrum.
费用和不便限制了过滤氙弧灯（太阳模拟器）作为紫外线光源用于大规模动物研究。由于防晒免疫保护水平会受到光源紫外线功率谱的显著影响，因此必须使用太阳模拟光源来准确测量与人类阳光紫外线暴露相关的防晒保护水平。然而，有相对便宜的太阳灯，例如UVA - 340，其发出的紫外线功率谱与太阳模拟器相似。与FS型UVB太阳灯不同，FS型UVB太阳灯在波长低于295nm时有大量有效的免疫抑制非太阳紫外线能量，UVA - 340太阳灯的免疫抑制效果光谱与太阳模拟器几乎相同。本研究的目的是评估这种太阳灯用于进行光免疫学和防晒免疫保护研究的情况。将C3H小鼠分组暴露于一系列UVA - 340太阳灯剂量（0.25kJ/m²至20.0kJ/m²），以建立剂量反应曲线并确定诱导局部型接触超敏反应（CH）抑制的最小免疫抑制剂量（MISD）。MISD定义为给予小鼠产生约50% CH反应抑制的最低紫外线剂量，对于UVA - 340太阳灯，MISD被确定为1.0kJ/m²。然后使用UVA - 340太阳灯以MISD为终点对四种市售防晒乳液（防晒系数[SPF] 4、8、15和30）进行免疫保护测试。这些防晒霜的免疫保护因子与它们标注的SPF所预测的保护水平相当。这些结果与我们之前使用太阳模拟器获得的结果相似。我们从这些数据得出结论，UVA - 340太阳灯的免疫抑制作用与太阳模拟器相似；然而，需要进一步研究以确定UVA - 340或类似的太阳灯是否是太阳模拟器的可行替代品，用于进行需要相关太阳紫外线光谱的大规模动物实验。
