[Reversal of malignant phenotype of cells of human pancreatic adenocarcinoma cell line by recombinant retroviral vector expressing antisense c-myc].

A recombinant retroviral vector expressing antisense c-myc was constructed. The recombinant virus DNA was packaged with packaging cell line PA317 cells by the calcium phosphate coprecipitation method. The virus supernatant was used to infect human pancreatic carcinoma cell line PC-2. After selection with G418, resistant colonies were obtained. Stable expression of retrovirus in both PA317 and PC-2 cells were confirmed by Northern blot hybridization. A down-regulation of endogenous c-myc was found in PC-2 cells infected with antisense c-myc construct. It was demonstrated that the antisense c-myc did inhibit the cell growth rate and 3H-TdR incorporation rate of PC-2 cells. The ability of colony formation in soft agar and tumorogenicity in nude mice of PC-2 cells were significantly suppressed by the antisense c-myc. The results implicate that recombinant retroviral vector containing antisense c-myc could inhibit target gene expression and partly reverse malignant phenotype of pancreatic adenocarcinoma cells.