[维甲酸和二甲基亚砜对HL-60细胞系及其耐药亚系中MDR 1基因表达的诱导作用以及对罗丹明-123外排的影响]

[Induction of expression of MDR 1 gene by retinoic acid and DMSO and effects on rhodamine-123 efflux in HL-60 cell lines and resistant sublines].

作者信息

Zhou W D, Zhang H Q, Fang M, He Q Y, Pang D B, Xue S B

机构信息

Department of Biology, Beijing Normal University.

出版信息

Yao Xue Xue Bao. 1996;31(1):1-5.

PMID:8762455

Abstract

Using dot blot hybridization and flowcytometry, the effects of differentiation inducers retinoic acid (RA) and dimethyl sulfoxide (DMSO) on the resistant level of HL-60 cells and its resistant subline cells were studied. When the cells were treated with RA 1 mumol.L-1 for 24 h, the expression of MDR 1 mRNA evidently increased in both HL-60 and its multidrug resistant subline cells. The efflux of Rho-123 in the multidrug resistant subline cells was slightly decreased. But, when the cells were treated with 2% DMSO for 24 h the efflux of Rho-123 increased obviously. The results suggest that RA can induce the expression of MDR1 gene but perhaps inhibit the function of pump glycoprotein 170 (Pgp-170) through phosphorylation/dephosphorylation pathway. However, DMSO could induce the expression of full function of Pgp.

摘要

采用斑点杂交和流式细胞术，研究了分化诱导剂维甲酸（RA）和二甲基亚砜（DMSO）对HL-60细胞及其耐药亚系细胞耐药水平的影响。当细胞用1μmol·L-1的RA处理24小时时，HL-60及其多药耐药亚系细胞中MDR 1 mRNA的表达明显增加。多药耐药亚系细胞中罗丹明123（Rho-123）的外排略有下降。但是，当细胞用2% DMSO处理24小时时，Rho-123的外排明显增加。结果表明，RA可诱导MDR1基因的表达，但可能通过磷酸化/去磷酸化途径抑制泵糖蛋白170（Pgp-170）的功能。然而，DMSO可诱导Pgp全功能的表达。