Sun L, Ba F, Liang P, Tian Y, Mei R, Mang K
Institute of Microbiology, Academia Sinica, Beijing.
Wei Sheng Wu Xue Bao. 1996 Feb;36(1):69-72.
The Bacillus thuringiensis toxin gene CryIA(c) was inserted into the shuttle vector pBE-2 to construct pAMY for expressing the B.t. gene in both Gram-negative and -positive bacterial systems. pAMY was introduced into wild type Bacillus cereus, B.brevis and B.subtilis by electroporation. Transformants containing delta-endotoxin gene produced proteins reacted with B.t. crystal protein antibody. Upon biological toxicity tests, the transformants gave a mortality of 100% against Ostrinia furnacilis, 58.8% against Heliothis armigera and 100% against Heliothis assulta. The ability of promoting plant growth of the original strains is retained.
将苏云金芽孢杆菌毒素基因CryIA(c)插入穿梭载体pBE-2中构建pAMY，以便在革兰氏阴性和阳性细菌系统中表达苏云金芽孢杆菌基因。通过电穿孔将pAMY导入野生型蜡状芽孢杆菌、短短芽孢杆菌和枯草芽孢杆菌。含有δ-内毒素基因的转化子产生的蛋白质能与苏云金芽孢杆菌晶体蛋白抗体发生反应。经生物毒性测试，这些转化子对亚洲玉米螟的致死率为100%，对棉铃虫的致死率为58.8%，对烟青虫的致死率为100%。原始菌株促进植物生长的能力得以保留。
