Egan C A, Bishop M A, Hector M P
Department of Anatomy, Queen Mary and Westfield College, London, UK.
J Anat. 1996 Jun;188 ( Pt 3)(Pt 3):623-31.
The innervation of pulp and dentine was studied in fully formed human deciduous teeth using antibodies to calcitonin gene related peptide (CGRP). Freshly extracted healthy teeth were divided, fixed, demineralised, cryosectioned and treated with antibodies to human CGRP which was then labelled with horseradish peroxidase. Bundles of nerve fibres passed from the apex of the root to the coronal region where a subodontoblast plexus was formed. In the cervical half of the root some nerve fibres branched away from the main bundles to supply both the odontoblast layer and the dentine. Branches from the coronal subodontoblast plexus also reached the odontoblast layer and the dentine. Most of the nerve fibres terminated in the odontoblast layer. In some areas a marginal plexus of nerves was observed between the odontoblasts and the predentine; intratubular nerve fibres arose either from this plexus or directly from the pulp. The dentine of the crown was more densely innervated than that of the root. In the crown the cervical one third had the most densely innervated dentine followed by the pulp horn and the middle third. The most densely innervated areas occurred in regions where the marginal plexus was present. Although many tubules contained a single nerve filament more complex patterns of termination were also observed. The maximum penetration of a nerve fibre into the dentine was 125 microns. The pattern of the deciduous innervation shows some similarities to the permanent dentition but among the differences is the high density of dentinal innervation in the cervical region. The latter point correlates with the clinical impression of greater sensitivity experienced by patients during invasive procedures performed without anaesthetic in the cervical area.
利用抗降钙素基因相关肽（CGRP）抗体，对完全形成的人乳牙牙髓和牙本质的神经支配进行了研究。新鲜拔除的健康牙齿被分割、固定、脱矿、冷冻切片，并用抗人CGRP抗体处理，然后用辣根过氧化物酶标记。神经纤维束从牙根尖延伸至冠部区域，在那里形成了成牙本质细胞下丛。在牙根颈段，一些神经纤维从主束分出，为成牙本质细胞层和牙本质提供营养。冠部成牙本质细胞下丛的分支也延伸至成牙本质细胞层和牙本质。大多数神经纤维在成牙本质细胞层终止。在某些区域，观察到成牙本质细胞和前期牙本质之间有边缘神经丛；管内神经纤维要么起源于该神经丛，要么直接来自牙髓。牙冠的牙本质比牙根的神经支配更密集。在牙冠中，颈三分之一的牙本质神经支配最密集，其次是髓角和中三分之一。神经支配最密集的区域出现在有边缘神经丛的部位。尽管许多牙本质小管含有单根神经细丝，但也观察到了更复杂的终末模式。神经纤维向牙本质内的最大穿透深度为125微米。乳牙的神经支配模式与恒牙列有一些相似之处，但不同之处在于颈区牙本质神经支配密度高。后一点与患者在颈部区域未麻醉的侵入性操作中感受到更高敏感性的临床印象相关。
