Tao R, Auerbach S B
Department of Biological Sciences, Rutgers University, New Brunswick, New Jersey, USA.
J Neurochem. 1996 Mar;66(3):1067-75. doi: 10.1046/j.1471-4159.1996.66031067.x.
The contribution of NMDA receptors to regulation of serotonin (5-HT) release was assessed by in vivo microdialysis in freely behaving rats. During infusion of NMDA (30, 100, and 300 microM) into the dorsal raphe nucleus (DRN), 5-HT was increased by approximately 25, 100, and 280%, respectively. Competitive and noncompetitive NMDA-receptor antagonists blocked this effect on DRN 5-HT. Infusion of NMDA (300 microM) into the DRN also produced an 80% increase in extracellular 5-HT in the nucleus accumbens. During infusion of NMDA (100 and 300 microM) into the median raphe nucleus (MRN), 5-HT was increased by approximately 15 and 80%, respectively. NMDA-receptor antagonists blocked this effect on MRN 5-HT. Infusion of NMDA into the MRN also produced a significant increase in hippocampal 5-HT. In contrast, infusion of NMDA into the nucleus accumbens, frontal cortex, or hippocampus produced small decreases in 5-HT in these forebrain sites. Taken together, these results suggest that NMDA receptors in the midbrain raphe, but not the forebrain, can have an excitatory influence on 5-HT neurons and, thus, produce increased 5-HT release in the forebrain. Furthermore, in comparison with the MRN, DRN 5-HT neurons were more sensitive to the excitatory effect of NMDA.
通过对自由活动大鼠进行体内微透析，评估了N-甲基-D-天冬氨酸（NMDA）受体对5-羟色胺（5-HT）释放调节的作用。向中缝背核（DRN）注入NMDA（30、100和300微摩尔）时，5-HT分别增加了约25%、100%和280%。竞争性和非竞争性NMDA受体拮抗剂可阻断对DRN 5-HT的这种作用。向DRN注入NMDA（300微摩尔）也使伏隔核细胞外5-HT增加了80%。向中缝正中核（MRN）注入NMDA（100和300微摩尔）时，5-HT分别增加了约15%和80%。NMDA受体拮抗剂可阻断对MRN 5-HT的这种作用。向MRN注入NMDA也使海马体5-HT显著增加。相比之下，向伏隔核、额叶皮质或海马体注入NMDA会使这些前脑部位的5-HT略有减少。综上所述，这些结果表明，中脑缝际核而非前脑中的NMDA受体可对5-HT能神经元产生兴奋性影响，从而使前脑中5-HT释放增加。此外，与MRN相比，DRN的5-HT能神经元对NMDA的兴奋作用更敏感。
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