Conde M, Montaño R, Moreno-Aurioles V R, Ramirez R, Sanchez-Mateos P, Sanchez-Madrid F, Sobrino F
Departamento de Bioquimica Medica y Biologia Molecular, Facultad de Medicina, Universidad de Sevilla, Spain.
J Leukoc Biol. 1996 Aug;60(2):278-84. doi: 10.1002/jlb.60.2.278.
The human activation antigen CD69 is an early inducible surface glycoprotein acquired by T cells in the thymus at the stage of positive selection and during activation of mature lymphoid cells both in vivo and in vitro. We have studied the regulatory influence of CD69 activation pathway on the glycolytic process and transduction signals of thymocytes. Treatment of human thymocytes with different anti-CD69 monoclonal antibodies (mAbs), in the presence of submitogenic doses of phorbol ester, produced an enhanced release of lactate without significant alterations in Fru 2,6-P2 levels or phosphofructokinase-2 (PFK-2) and pyruvate kinase activities. A small increase in phosphofructokinase-1 (PFK-1) activity was also detected. Furthermore, anti-CD69 mAb increased the glucose detritiation from [2-3H] and [3-3H]glucose, thus indicating an enhanced flux through hexokinase and PFK-1 steps. In addition, de novo synthesis of diacylglycerol and intracellular Ca2+ levels increased after anti-CD69 mAb treatment. The stimulatory effects of anti-CD69 mAb on both glycolysis and Ca2+ levels were inhibited by cyclosporin A. Because CD69 molecules are present in certain subset populations of immature thymocytes, the ability of anti-CD69 mAb to stimulate the glycolysis, the synthesis of diacylglycerol and the intracellular Ca2+ levels suggest that the activation signals delivered through CD69 molecules could play a role in the thymus cells maturation.
人类激活抗原CD69是一种早期可诱导的表面糖蛋白，在阳性选择阶段的胸腺中以及体内外成熟淋巴细胞激活过程中，T细胞均可获得该蛋白。我们研究了CD69激活途径对胸腺细胞糖酵解过程和转导信号的调节作用。在亚致有丝分裂剂量的佛波酯存在下，用不同的抗CD69单克隆抗体（mAb）处理人胸腺细胞，可使乳酸释放增加，而果糖2,6-二磷酸（Fru 2,6-P2）水平、磷酸果糖激酶-2（PFK-2）和丙酮酸激酶活性无明显改变。还检测到磷酸果糖激酶-1（PFK-1）活性略有增加。此外，抗CD69 mAb增加了[2-³H]葡萄糖和[3-³H]葡萄糖的葡萄糖脱氚作用，从而表明通过己糖激酶和PFK-1步骤的通量增加。另外，抗CD69 mAb处理后二酰基甘油的从头合成和细胞内Ca²⁺水平升高。抗CD69 mAb对糖酵解和Ca²⁺水平的刺激作用被环孢素A抑制。由于CD69分子存在于未成熟胸腺细胞的某些亚群中，抗CD69 mAb刺激糖酵解、二酰基甘油合成和细胞内Ca²⁺水平的能力表明，通过CD69分子传递的激活信号可能在胸腺细胞成熟中起作用。
