Sugiyama H, Hatano K, Saito I, Amontov S, Taira K
Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Japan.
FEBS Lett. 1996 Sep 2;392(3):215-9. doi: 10.1016/0014-5793(96)00814-9.
A minizyme is a hammerhead ribozyme with short oligonucleotide linkers instead of stem/loop II. In a previous study we demonstrated that a minizyme with high-level activity forms a dimeric structure with a common stem II (Amontov and Taira, J. Am. Chem. Soc., 118 (1996) 1624-1628). As a continuation of this study, we recently examined whether a short oligonucleotide linker at stem/loop II could be replaced by a triterpenoid linker and whether the resulting minizymes with bulky hydrophobic groups would form dimeric structures. In contrast to the minizyme with high-level activity that we characterized in the previous study, minizymes that contained a triterpenoid group had low cleavage activities. The nature of the dependence of the activity on the concentration of ribozyme revealed that these minizymes with a triterpenoid group do not form dimeric structures. Thus, the low activities of these structures can be attributed to their failure to form dimers.
微小酶是一种锤头状核酶，其茎环II被短寡核苷酸接头取代。在之前的一项研究中，我们证明具有高活性的微小酶会形成带有共同茎II的二聚体结构（阿蒙托夫和 Taira，《美国化学会志》，118 (1996) 1624 - 1628）。作为这项研究的延续，我们最近研究了茎环II处的短寡核苷酸接头是否可以被三萜类接头取代，以及由此产生的带有庞大疏水基团的微小酶是否会形成二聚体结构。与我们在之前研究中表征的具有高活性的微小酶不同，含有三萜类基团的微小酶具有较低的切割活性。活性对核酶浓度的依赖性本质表明，这些带有三萜类基团的微小酶不会形成二聚体结构。因此，这些结构的低活性可归因于它们未能形成二聚体。
