Yap I, Chan S H
Department of Medicine, National University of Singapore, Singapore.
Ann Acad Med Singap. 1996 Jan;25(1):120-2.
Nine healthy subjects who had no detectable antibody response when initially vaccinated with hepatitis B vaccine (plasma derived and/or yeast derived) were revaccinated with three 20 micrograms doses of a new recombinant hepatitis B (HB) vaccine (SCI-B-VAC) derived from Chinese hamster ovary (CHO) cells and consisting of both the major S protein and the minor pre-S (S1 and S2) proteins of the viral coat. Seroconversion was successful in 78% of these non-responders after the second dose and just before the third vaccination. The geometric mean titres of the antiHBs were 587 iu/l one month after the course of vaccination. This study, though preliminary, indicated that pre-S containing CHO vaccine is effective in inducing antibody response in S-vaccine non-responders and also suggested that the added pre-S region in HBV vaccine helps antiHBs production. Frequency of HLA B46 and B15 were higher in non-responders compared to normal subjects (P = 0.04) supporting the possibility that genetic factors may modulate the immune response to HB vaccination.
9名最初接种乙肝疫苗（血浆源性和/或酵母源性）时未检测到抗体反应的健康受试者，用3剂每剂20微克的新型重组乙肝（HB）疫苗（SCI-B-VAC）进行再接种，该疫苗由中国仓鼠卵巢（CHO）细胞衍生，包含病毒衣壳的主要S蛋白和次要前S蛋白（S1和S2）。在第二次接种后且恰好在第三次接种前，这些无反应者中有78%成功实现了血清转化。接种疗程结束后1个月，抗HBs的几何平均滴度为587 iu/l。本研究虽为初步研究，但表明含前S的CHO疫苗在诱导S疫苗无反应者产生抗体反应方面有效，还提示乙肝疫苗中添加的前S区域有助于抗HBs的产生。与正常受试者相比，无反应者中HLA B46和B15的频率更高（P = 0.04），这支持了遗传因素可能调节对乙肝疫苗免疫反应的可能性。
