阿卡波糖产生菌游动放线菌属的无细胞提取物形成磷酸阿卡波糖

Formation of acarbose phosphate by a cell-free extract from the acarbose producer Actinoplanes sp.

作者信息

Goeke K, Drepper A, Pape H

机构信息

Institut für Mikrobiologie, Westfälische Wilhelms-Universität, Münster, Germany.

出版信息

J Antibiot (Tokyo). 1996 Jul;49(7):661-3. doi: 10.7164/antibiotics.49.661.

DOI:10.7164/antibiotics.49.661

PMID:8784426

Abstract

The alpha-glucosidase inhibitor acarbose is modified during incubation with cell-free extract from the producing Actinoplanes strain. The formation of this product depends on the presence of ATP. Chromatographic and chemical properties of the purified transformation product indicate the presence of a phosphate ester. The structure is deduced by NMR analysis and shown to be acarbose-7-phosphate.

摘要

α-葡萄糖苷酶抑制剂阿卡波糖在与产生它的游动放线菌菌株的无细胞提取物温育过程中会发生修饰。该产物的形成依赖于ATP的存在。纯化的转化产物的色谱和化学性质表明存在磷酸酯。通过核磁共振分析推导其结构，结果显示为阿卡波糖-7-磷酸。