Ha C L, Paulino-Racine L E, Woodward B D
Department of Human Biology and Nutritional Sciences, University of Guelph, Canada.
Br J Nutr. 1996 Mar;75(3):445-60. doi: 10.1079/bjn19960146.
A direct comparison of systemic (spleen) and mucosal (intestine) antibody-producing systems was made in weanling male C57BL/6J mice subjected to wasting protein-energy malnutrition (PEM) by means of a low-protein protocol known to duplicate immunological and physiological features of human malnutrition. ELISA revealed low concentrations of biliary and gut lumen immunoglobulin (Ig) A in malnourished mice concomitantly with a high concentration of blood IgA. The low-protein model, therefore, exhibited fidelity to human protein-energy malnutrition in its influence on the concentrations of the mucosal Ig, IgA, in critical biological fluids. The number of IgA-, IgM- and IgG-containing cells was estimated morphometrically on a per organ basis. The low-protein protocol supported expansion in numbers of mucosal IgA-containing cells (18 x relative to a zero-time control group) and of splenic IgG-containing cells (135x), albeit an attenuated expansion in comparison with that of well-nourished control animals (132x and 571x respectively relative to zero-time controls). Up to terminal differentiation of Ig-containing cells, systemic and mucosal antibody-producing systems exhibited similarly remarkable resistance to wasting malnutrition. Epithelial transport of IgA may be an aspect of the mucosal antibody response which is particularly sensitive to PEM.
通过一种已知可复制人类营养不良免疫和生理特征的低蛋白方案,对断奶雄性C57BL/6J小鼠进行消瘦型蛋白质-能量营养不良(PEM)处理,从而对全身(脾脏)和黏膜(肠道)抗体产生系统进行了直接比较。酶联免疫吸附测定(ELISA)显示,营养不良小鼠的胆汁和肠腔免疫球蛋白(Ig)A浓度较低,而血液IgA浓度较高。因此,低蛋白模型在对关键生物体液中黏膜Ig(即IgA)浓度的影响方面,表现出与人类蛋白质-能量营养不良的相似性。基于每个器官,通过形态计量学估计含IgA、IgM和IgG细胞的数量。低蛋白方案促使黏膜含IgA细胞数量增加(相对于零时对照组增加了18倍)以及脾脏含IgG细胞数量增加(增加了135倍),尽管与营养良好的对照动物相比,这种增加有所减弱(相对于零时对照组,分别为132倍和571倍)。直至含Ig细胞的终末分化,全身和黏膜抗体产生系统对消瘦型营养不良均表现出同样显著的抗性。IgA的上皮转运可能是黏膜抗体反应中对PEM特别敏感的一个方面。